A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in arcuate nucleus.
A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in arcuate nucleus.
复制标题
对弓状核中 GABA 能神经元的强大 GABA(B) 受体介导抑制。
DOI：
10.1097/00001756-199908200-00045
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发表时间：
1999
期刊：
影响因子：
1.7
通讯作者：
Rønnekleiv,OK
中科院分区：
文献类型：
--
作者：
Wagner,EJ;Bosch,MA;Kelly,MJ;Rønnekleiv,OK
WE combined histofluorescence with in situ hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recording, using GAD 65 as a marker. Intracellular recordings 91 were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA B receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose dependent, and the GABA B receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose–response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA B receptor activation between GABAergic and non-GABAergic ARC neurons.
