Effect of the HBV whole-X gene on the expression of hepatocellular carcinoma associated proteins.
Effect of the HBV whole-X gene on the expression of hepatocellular carcinoma associated proteins.
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DOI：
10.1016/j.jmii.2014.07.004
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Yu Zhang;Hong-li Liu;Meiling Cui;Jinfeng Liu;R. Yi;Y. Niu;Tianyan Chen;Ying‐ren Zhao
中科院分区：
文献类型：
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作者：
Yu Zhang;Hong-li Liu;Meiling Cui;Jinfeng Liu;R. Yi;Y. Niu;Tianyan Chen;Ying‐ren Zhao
背景B型肝炎病毒（HBV）前X基因位于HBV X基因的上游，共同构成HBV全X基因。虽然它已被证明，HBV全-X蛋白参与肝细胞癌的发展，其生物学作用和分子机制仍然在很大程度上unknown.MethodsIn这项研究中，我们亚克隆HBV全-X基因，并构建了HBV全-X表达载体。将HBV全X基因转染HL-7702细胞后，采用双向电泳结合基质辅助激光解吸电离-飞行时间质谱（matrix-assisted laser desorption/ionization-time of flight mass spectrometry.ResultsThe结果显示，在转染或未转染HBV全X基因的细胞中，有18种主要蛋白质差异表达。这些基因的表达进一步证实了逆转录-聚合酶链反应和Western blot analysis.ConclusionOur的研究结果提供了一个新的见解调查的病理作用，HBV全X基因在肝细胞癌的发展，并可能导致设计新的策略治疗这种疾病。
BackgroundThe hepatitis B virus (HBV) pre-X gene resides upstream of the HBV X gene, and together they form the HBV whole-X gene. Although it has been evident that the HBV whole-X protein is involved in the development of hepatocellular carcinoma, its biological role and molecular mechanism remain largely unknown.MethodsIn this study, we subcloned the HBV whole-X gene and constructed a HBV whole-X expressing vector. After transfection of the HBV whole-X gene into HL-7702 cells, the profile of the differential cellular protein composition in the cells was analyzed by using two-dimensional electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry.ResultsThe results showed that 18 major proteins were differentially expressed in the cells transfected with or without the HBV whole-X gene. The expression of these genes was further confirmed by reverse transcription-polymerase chain reaction and Western blot analysis.ConclusionOur findings provide a new insight into the investigation of the pathological role that the HBV whole-X gene plays in the development of hepatocellular carcinoma and may lead to the design of novel strategies for the treatment of this disease.