An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography
An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography
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DOI：
10.1038/s41522-020-0129-y
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发表时间：
2020-04-01
影响因子：
9.2
通讯作者：
Wagner, Michael
中科院分区：
文献类型：
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作者：
Gierl, Luisa;Stoy, Kasper;Wagner, Michael
本文介绍了一种用于研究重复实验中生物膜发育的全自动培养和监测工具。为了从根本上了解培养条件和生物膜特性(如结构、机械)之间的关系，需要一个允许方法标准化的监测装置。光学相干层析成像(OCT)是一种理想的生物膜成像方式，因为它允许实时监测结构。通过将OCT设备集成到开源机器人平台EvoBot中，实现了一个同时研究多个流动细胞中生物膜发育的全自动化监测平台。对不同的定位方案进行了测试，结果表明定位精度在OCT的光学分辨率范围内。因此，通过OCT对生物膜发育进行可靠、准确的监测已成为可能。有了这个机器人平台，只需投入少量的操作员时间，就可以完成包括统计分析在内的可重复生物膜实验。此外，在这项研究中计算的一些结构参数证实了进行复制生物膜培养的必要性。
The paper introduces a fully automated cultivation and monitoring tool to study biofilm development in replicate experiments operated in parallel. To gain a fundamental understanding of the relation between cultivation conditions and biofilm characteristics (e.g., structural, mechanical) a monitoring setup allowing for the standardization of methods is required. Optical coherence tomography (OCT) is an imaging modality ideal for biofilms since it allows for the monitoring of structure in real time. By integrating an OCT device into the open-source robotic platform EvoBot, a fully automated monitoring platform for investigating biofilm development in several flow cells at once was realized. Different positioning scenarios were tested and revealed that the positioning accuracy is within the optical resolution of the OCT. On that account, a reliable and accurate monitoring of biofilm development by means of OCT has become possible. With this robotic platform, reproducible biofilm experiments including a statistical analysis are achievable with only a small investment of operator time. Furthermore, a number of structural parameters calculated within this study confirmed the necessity to perform replicate biofilm cultivations.