Bioavailability and catabolism of green tea flavan-3-ols in humans
Bioavailability and catabolism of green tea flavan-3-ols in humans
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DOI：
10.1016/j.nut.2009.09.021
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发表时间：
2010-11-01
期刊：
影响因子：
4.4
通讯作者：
Brighenti, Furio
中科院分区：
文献类型：
--
作者：
Del Rio, Daniele;Calani, Luca;Brighenti, Furio
目的：本研究的目的是通过高效液相色谱串联质谱法研究绿色茶黄烷-3-醇催化剂和血浆药代动力学和尿排泄，以评估其绝对生物利用度，同时考虑到所有已知和一些未知的催化剂与胃肠道及其宿主微生物菌群的相互作用。在20名健康志愿者中进行喂养研究，这些志愿者摄入400 mL含有约400 μ mol黄烷-3-醇的即饮绿色茶。分别收集4 h和24 h的尿液和血浆，通过串联质谱法在这些生物体液中鉴定出39种相关的黄烷-3-醇催化剂。在血浆中，（-）-表没食子儿茶素-3-没食子酸酯是唯一未代谢的化合物，与（-）-表没食子儿茶素和（-）-表儿茶素结合物相比，绝对浓度最高。结肠微生物衍生的聚羟基苯基-γ-戊内酯是目前主要的尿中catenin，平均浓度比黄烷-3-醇结合物高10倍。计算的生物利用度等于39%，有趣的是，注意到参与者之间结肠代谢物的尿液排泄存在很大的差异，可能与他们自己的结肠微生物区系差异有关。结论：这项研究表明，绿色茶儿茶素比以前观察到的结肠环分裂代谢物时，考虑到生物利用度。经常食用含有黄烷-3-醇的即饮绿色茶允许人体非边际暴露于这些catenomers，以某种方式证明了与绿色茶摄入量相关的许多有益作用。(C)2010年爱思唯尔公司All rights reserved.
Objective: The aim of this study was to investigate green tea flavan-3-ol catabolism and plasma pharmacokinetic and urinary excretion by high-performance liquid chromatography with tandem mass spectrometry to evaluate their absolute bioavailability by taking into account all known and some unknown catabolites deriving from their interaction with the gastrointestinal tract and its host microflora.Methods: A feeding study was carried out in 20 healthy human volunteers who ingested 400 mL of a ready-to-drink green tea containing approximately 400 mu mol of flavan-3-ols. Urine and plasma were collected for 4 and 24 h, respectively, and 39 relevant catabolites were identified in these biological fluids by tandem mass spectrometry.Results: In biological fluids, 39 relevant flavan-3-ol catabolites were identified. In plasma, (-)-epigallocatechin-3-gallate was the only unmetabolized compound and the highest in absolute concentration compared with (-)-epigallocatechin and (-)-epicatechin conjugates. Colonic microflora-derived polyhydroxyphenyl-gamma-valerolactones were by far the main urinary catabolites, averaging 10 times greater concentratin than flavan-3-ol conjugates. The calculated bioavailability was equal to 39% and it is interesting to notice the great variability in urinary excretion of colonic metabolites among participants, probably related to differences in their own colonic microflora. Conclusion: This study demonstrates that green tea catechins are more bioavailable than previously observed when colonic ring fission metabolites are taken into consideration. Regular consumption of ready-to-drink green tea containing flavan-3-ols allows a non-marginal exposure of the human body to these catabolites, somehow justifying the numerous beneficial actions described as linked to green tea intake. (C) 2010 Elsevier Inc. All rights reserved.