Human astrovirus isolation and propagation in multiple cell lines
Human astrovirus isolation and propagation in multiple cell lines
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DOI：
10.1007/s007050070060
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发表时间：
2000-01-01
影响因子：
2.7
通讯作者：
Herrmann, JE
中科院分区：
文献类型：
--
作者：
Brinker, JP;Blacklow, NR;Herrmann, JE
对实验室适应人类星状病毒血清型1至7在15个人类、7个猿类和10个其他非灵长类哺乳动物细胞系中的生长情况进行了测试。所有7种血清型在人类细胞系Caco-2、T84、HT-29和非洲绿猴肾细胞系MA-104中繁殖成功。原代和继代非洲绿猴肾细胞培养星状病毒的效果均优于恒河猴肾细胞。除了人类包皮细胞外，所有其他人类和类人猿细胞系都支持至少一种星状病毒血清型的生长。唯一允许星状病毒持续传代的非灵长类细胞系是用于星状病毒血清型2的BHK-21 （C13）细胞系。在Caco-2、T84、HT-29、SK-CO-I、PLC/PRF/5、MA-104和VERO细胞中培养17例先前经ELISA检测为星状病毒阳性的人粪便标本。Caco-2细胞（13株）、T84细胞（12株）和PLC/PRF/5细胞（12株）是从临床粪便标本中分离人类星状病毒最有效的细胞系。通过感染细胞的免疫荧光染色，同样17个标本在壳小瓶中培养18 h， Caco-2细胞中17个标本的星状病毒阳性，T84细胞中12个，PLC/PRF/5细胞中7个。壳瓶法是一种快速、灵敏的星状病毒检测方法。
Laboratory adapted human astrovirus serotypes 1 through 7 were tested for growth in 15 human, 7 simian, and 10 other non-primate mammalian cell lines. Propagation of all seven serotypes was successful in the human cell lines Caco-2, T84, HT-29, and in the African green monkey kidney cell line MA-104. Both primary and secondary African green monkey kidney cells were more effective than Rhesus monkey kidney cells for cultivation of astrovirus. Except for human foreskin cells, all of the other human and simian cell lines supported growth of at least one astrovirus serotype. The only non-primate cell line that permitted sustained passage of astroviruses was the BHK-21 (C13) cell line for astrovirus serotype 2. Seventeen human stool specimens that had previously been shown to be astrovirus positive by ELISA were cultured in Caco-2, T84, HT-29, SK-CO-I, PLC/PRF/5, MA-104, and VERO cells. Caco-2 cells (13 isolates), T84 cells (12 isolates) and PLC/PRF/5 cells (12 isolates) were the cell lines most effective for isolation of human astroviruses from clinical stool specimens. By immunofluorescent staining of infected cells, culturing of the same 17 specimens in shell vials for 18 h was positive for astroviruses in all 17 specimens in Caco-2 cells, 12 in T84 cells, and 7 in PLC/PRF/5 cells. Shell vial assay is suitable as a rapid and sensitive culture technique for detection of astroviruses in clinical specimens.