Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Megalobrama amblycephala Fed High-Carbohydrate Diets by Activating the AMPK-SIRT1-PGC-1α Network.
Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Megalobrama amblycephala Fed High-Carbohydrate Diets by Activating the AMPK-SIRT1-PGC-1α Network.
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白藜芦醇通过激活 AMPK-SIRT1-PGC-1α 网络改善高碳水化合物饮食的团头鲂的能量感应和糖脂代谢
DOI：
10.3389/fphys.2018.01258
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发表时间：
2018
影响因子：
4
通讯作者：
Li XF
中科院分区：
文献类型：
--
作者：
Shi HJ;Xu C;Liu MY;Wang BK;Liu WB;Chen DH;Zhang L;Xu CY;Li XF
本研究调查了白藜芦醇对高碳水化合物饮食的团头鲂的生长性能、能量感应、糖脂代谢以及葡萄糖和胰岛素负荷的影响。鱼（39.44±0.06克）被随机喂食三种饮食：对照饮食（30%碳水化合物）、高碳水化合物饮食（HC，41%碳水化合物）和补充0.04%白藜芦醇（HCR）的HC饮食，持续12周。与对照组相比，饲喂HC饲料的鱼的氮和能量保留效率、肝体指数、腹腔内脂肪比、全身脂质含量以及腹腔内脂肪糖原和脂质含量均显着升高，但与HCR处理组差异不大。饲喂HC饲料的鱼的肝脏和肌肉脂质含量以及血浆葡萄糖、糖化血清蛋白、晚期糖基化终末产物和总胆固醇的水平显着高于对照组，而补充白藜芦醇则相反。与 HCR 处理相比，饲喂 HC 饲料的鱼的血浆胰岛素水平、肝单磷酸腺苷 (AMP) 含量和 NAD+/NADH 比值显着降低，但与对照组差异不大。肝三磷酸腺苷 (ATP) 含量和 ATP/AMP 比值则相反。此外，与对照组相比，饲喂 HC 饲料的鱼的葡萄糖转运蛋白 2 (GLUT2)、葡萄糖 6-磷酸脱氢酶、糖原合成酶、脂肪酸合成酶 (FAS)、乙酰辅酶 A 羧化酶 α (ACCα)、过氧化物酶体增殖物激活受体 γ 和 PPARα 的转录水平显着高于对照组，而 AMP 激活蛋白激酶 α (t-AMPKα) 的蛋白质水平则相反。磷酸化 AMP 激活蛋白激酶 α (p-AMPKα)、sirtuin-1 (SIRT1) 和 p-AMPKα/t-AMPKα 比率以及 AMPKα1、AMPKα2、SIRT1、PPARγ 共激活剂-1α (PGC-1α)、磷酸烯醇丙酮酸羧激酶、果糖-1,6-双磷酸酶 (FBPase) 的转录，葡萄糖-6-磷酸酶、肉毒碱棕榈酰转移酶 I (CPT I) 和酰基辅酶 A 氧化酶。与HC组相比，补充白藜芦醇显着上调了t-AMPK、p-AMPK和SIRT1的蛋白水平、p-AMPK/t-AMPK比率以及AMPKα1、AMPKα2、SIRT1、PGC-1α、GLUT2、FBPase和CPT I的转录，而甾醇调节元件结合蛋白-1、FAS和ACCα的情况相反。此外，白藜芦醇改善了在葡萄糖和胰岛素负荷后喂食 HC 饮食的鱼的葡萄糖和胰岛素耐受性。
This study investigated the effects of resveratrol on the growth performance, energy sensing, glycolipid metabolism and glucose and insulin load of blunt snout bream Megalobrama amblycephala fed high-carbohydrate diets. Fish (39.44 ± 0.06 g) were randomly fed three diets: a control diet (30% carbohydrate), a high-carbohydrate diet (HC, 41% carbohydrate), and the HC diet supplemented with 0.04% resveratrol (HCR) for 12 weeks. Fish fed the HC diet had significantly high values of nitrogen and energy retention efficiency, hepatosomatic index, intraperitoneal fat ratio, whole-body lipid content and intraperitoneal fat glycogen and lipid contents compared to the control group, but showed little difference with the HCR treatment. Liver and muscle lipid contents and plasma levels of glucose, glycated serum protein, advanced glycation end products and total cholesterol of fish fed the HC diet were significantly higher than those of the control group, whereas the opposite was found with resveratrol supplementation. Fish fed the HC diet obtained significantly low values of plasma insulin levels and hepatic adenosine monophosphate (AMP) contents and NAD+/NADH ratio compared to HCR treatment, but showed little difference with the control group. The opposite was found for hepatic adenosine triphosphate (ATP) contents and the ATP/AMP ratio. In addition, fish fed the HC diet showed significantly high transcriptions of glucose transporter 2 (GLUT2), glucose-6-phosphate dehydrogenase, glycogen synthase, fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), peroxisome proliferator-activated receptor γ and PPARα compared to the control group, whereas the opposite was found for protein levels of AMP-activated protein kinase α (t-AMPKα), phosphorylated AMP-activated protein kinase α (p-AMPKα), sirtuin-1 (SIRT1), and p-AMPKα/t-AMPKα ratio as well as the transcriptions of AMPKα1, AMPKα2, SIRT1, PPARγ coactivator-1α (PGC-1α), phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase (FBPase), glucose-6-phosphatase, carnitine palmitoyl transferase I (CPT I) and acyl-CoA oxidase. Resveratrol supplementation significantly up-regulated the protein levels of t-AMPK, p-AMPK, and SIRT1, p-AMPK/t-AMPK ratio as well as the transcriptions of AMPKα1, AMPKα2, SIRT1, PGC-1α, GLUT2, FBPase, and CPT I compared to HC group, while the opposite was found for sterol regulatory element-binding protein-1, FAS and ACCα. Furthermore, resveratrol improved glucose and insulin tolerance of fish fed the HC diet after glucose and insulin load.
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影响因子：
7.1
作者：
Fischer-Posovszky, Pamela;Kukulus, Vera;Wabitsch, Martin
通讯作者：
Wabitsch, Martin
DOI：
10.1016/j.bbrc.2008.06.104
发表时间：
2008-09-12
影响因子：
3.1
作者：
Breen, Danna M.;Sanli, Toran;Tsiani, Evangelia
通讯作者：
Tsiani, Evangelia
DOI：
10.1152/ajpgi.90358.2008
发表时间：
2008-10-01
影响因子：
4.5
作者：
Ajmo, Joanne M.;Liang, Xiaomei;You, Min
通讯作者：
You, Min
杂食性 GIFT 罗非鱼 Oreochromis niloticus 葡萄糖负荷后前胰岛素原表达、胰岛素释放和肝脏葡萄糖代谢
影响因子：
4.5
作者：
Chen, Yong-Jun;Wang, Xin-Ya;Wang, De-Shou
通讯作者：
Wang, De-Shou
影响因子：
4.4
作者：
Cantó C;Auwerx J
通讯作者：
Auwerx J