First transcriptional survey of the Malpighian tubules of giant mealworm, Zophobas morio (Coleoptera: Tenebrionidae).
First transcriptional survey of the Malpighian tubules of giant mealworm, Zophobas morio (Coleoptera: Tenebrionidae).
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DOI：
10.4238/2015.january.23.21
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发表时间：
2015-01
期刊：
影响因子：
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通讯作者：
J. R. Silva;R. Prado;D. Amaral;V. Viviani
中科院分区：
文献类型：
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作者：
J. R. Silva;R. Prado;D. Amaral;V. Viviani
马氏管在昆虫渗透调节中起着关键作用。虽然已经对果蝇的马氏管进行了转录分析，但还没有对任何鞘翅目物种进行功能基因组学分析。最近，我们从赤霉菌的近缘幼虫Zobobas morio的Malighian小管中构建了一个cDNA文库，并克隆了一个具有荧光素酶样酶性质的AMP/CoA连接酶的cDNA。利用该文库，我们随机分离、部分测序和分析了约540个克隆，获得了最具代表性的表达基因的第一转录谱，并将它们与可能的生物学功能联系起来。发现高比例的线粒体基因，这与该器官在初级尿液形成过程中所需的高代谢活动相一致。常见的转录本包括参与渗透调节的酶，如溶质转运蛋白和ATPase，以及参与解毒和排泄的酶，如细胞色素P450，谷胱甘肽S转移酶，酒精脱氢酶。AMP/CoA连接酶的存在激活了外源羧酸，如萤火虫D-荧光素，表明它们参与了马氏管生理中新的异物排泄/解毒作用。
The Malpighian tubules play a key role in insect osmoregulation. Although a transcriptional analysis has been done for the Malpighian tubules in Drosophila melanogaster (Diptera), no functional genomics analysis has yet been carried out for any Coleoptera species. Recently, we constructed a cDNA library from Malpighian tubules of larval Zophobas morio, a close relative of Tribolium castaneum, and cloned the cDNA for an AMP/CoA-ligase with luciferase-like enzyme properties. Using this cDNA library, we randomly isolated, partially sequenced and analyzed ca. 540 clones, obtaining the first transcriptional profile of the most representative expressed genes, and associated them with their possible biological functions. A high percentage of mitochondrial genes was found, which is consistent with the high metabolic activity required by this organ during the formation of primary urine. Common transcripts included those for enzymes involved in osmoregulation, such as solute transporters and ATPases, and in detoxification and excretion, such as cytochrome P450, glutathione S-transferase, alcohol dehydrogenase. The presence of AMP/CoA-ligases, which activate exogenous carboxylic acids such as firefly D-luciferin suggests their participation in important new xenobiotic excretion/detoxification roles in Malpighian tubule physiology.