Ultrahigh-resolution optical coherence elastography
Ultrahigh-resolution optical coherence elastography
复制标题
DOI：
10.1364/ol.41.000021
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.6
通讯作者：
Sampson, David D.
中科院分区：
文献类型：
--
作者：
Curatolo, Andrea;Villiger, Martin;Sampson, David D.
在细胞和亚细胞水平上观察局部组织环境内的硬度有望提供对疾病发生和进展的洞察。在这封信中，我们提出了超高分辨率光学相干弹性成像（UHROCE），并展示了三维成像的局部轴向应变的组织进行压缩负荷。我们结合联合收割机光学相干显微镜（OCM）和相敏检测局部组织位移产生应变弹性图的分辨率（x，y，z）为2 × 2 × 15 μ m。我们证明了这一性能在一个新鲜切除的小鼠主动脉，并揭示了血管平滑肌细胞和弹性蛋白片的机械异质性，否则在一个典型的，较低分辨率的光学相干弹性成像（OCE）系统未解决。(C)2015美国光学学会
Visualizing stiffness within the local tissue environment at the cellular and subcellular level promises to provide insight into the genesis and progression of disease. In this Letter, we propose ultrahigh-resolution optical coherence elastography (UHROCE), and demonstrate 3D imaging of local axial strain of tissues undergoing compressive loading. We combine optical coherence microscopy (OCM) and phase-sensitive detection of local tissue displacement to produce strain elastograms with resolution (x,y,z) of 2 x 2 x 15 mu m. We demonstrate this performance on a freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography (OCE) system. (C) 2015 Optical Society of America