MORPHOGENETIC INTERACTIONS IN THE DEVELOPMENT OF THE MOUSE THYMUS GLAND
MORPHOGENETIC INTERACTIONS IN THE DEVELOPMENT OF THE MOUSE THYMUS GLAND
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DOI：
10.1016/0012-1606(60)90009-9
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发表时间：
1960-01-01
影响因子：
2.7
通讯作者：
AUERBACH, R
中科院分区：
文献类型：
--
作者：
AUERBACH, R
通过体外和移植方法对12天小鼠胚胎胸腺的形态发生进行了分析。在玻璃 - 血块界面，腺体生长并呈现出特征性的分叶，但主要仍为上皮性。在移植到成年小鼠眼前房时，它大量生长并变得具有淋巴样特性。通过使用胰蛋白酶，腺体可分离为上皮部分和间充质部分。这两种成分在体外或体内单独都不能发育，但是这两种组织聚集会导致形态发生系统的恢复，从而在体外产生分叶，在体内形成淋巴样组织。来自多种胚胎原基的间充质在体外可产生形态发生作用，上皮反应的具体模式因所用间充质的来源而异。间充质的影响可通过20微米的微孔滤膜屏障发挥作用。
Morphogenesis of 12-day mouse embryo thymus glands has been analyzed by in vitro and transplantation methods. At a glass-clot interface, the gland grows and undergoes characteristic lobulation, but remains predominantly epithelial. In transplants to the anterior chamber of adult mouse eyes it grows profusely and becomes lymphoidal. The gland may be separated into epithelial and mesenchymal portions by the use of trypsin. Neither component can develop in isolation, in vitro or in vivo,but clustering of the 2 tissues leads to the restoration of the morphogenetic system, with resultant in vitro lobulation and the in vivo formation of lymphoidal tissue. Mesenchyme from a variety of embryonic rudiments can exert a morphogenetic effect in vitro, the specific pattern of the epithelial response varying with the source of mesenchyme used. The mesenchymal influence can be exerted across a 20 [mu] millipore filter barrier.