Characterization of RNA interference in an Anopheles gambiae cell line
Characterization of RNA interference in an Anopheles gambiae cell line
复制标题
DOI：
10.1016/s0965-1748(03)00101-2
复制
发表时间：
2003-09-01
影响因子：
3.8
通讯作者：
Zheng, L
中科院分区：
文献类型：
--
作者：
Hoa, NT;Keene, KM;Zheng, L
将双链RNA引入无脊椎动物细胞中通常通过称为RNA干扰（RNAi）的机制导致靶基因的转录后沉默。双链RNA被称为dicer的RNA酶III样酶切割成小干扰RNA（siRNA）。在果蝇中，这些siRNA被掺入RNA诱导沉默复合物（RISC）中并介导靶mRNA的降解。RISC复合物含有Argonaute（Ago）蛋白质家族的成员。我们在这里显示，在冈比亚按蚊，在非洲的主要疟疾载体的血细胞系中的RNAi，需要表达dicer-2，Ago 2和Ago 3蛋白。此外，我们证明了蚊子中的RNAi不会扩散到靶区域之外，这表明蚊子中不存在RNA依赖的RNA聚合酶介导的传递性扩增。(C)2003 Elsevier Ltd.保留所有权利。
Introduction of double stranded RNA into invertebrate cells often results in posttranscriptional silencing of target genes through a mechanism termed RNA interference (RNAi). Double-stranded RNA is cleaved by an RNAse III-like enzyme, termed dicer, to small interfering RNAs (siRNAs). In Drosophila, these siRNAs are incorporated in the RNA induced silencing complex (RISC) and mediate degradation of target mRNA. The RISC complex contains members of Argonaute (Ago) family of proteins. We show here that RNAi in a hemocyte cell line of Anopheles gambiae, the principal malaria vector in Africa, requires expression of dicer-2, Ago2 and Ago3 proteins. Furthermore, we demonstrate that RNAi in the mosquito does not spread outside of the target region, suggesting that RNA dependent RNA polymerase mediated transitive amplification is absent in the mosquito. (C) 2003 Elsevier Ltd. All rights reserved.