DIVERGENT MESSENGER-RNA TRANSCRIPTION IN THE CHLOROPLAST PSBB OPERON
DIVERGENT MESSENGER-RNA TRANSCRIPTION IN THE CHLOROPLAST PSBB OPERON
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DOI：
10.1002/j.1460-2075.1988.tb02892.x
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发表时间：
1988-04-01
期刊：
影响因子：
11.4
通讯作者：
OHYAMA, K
中科院分区：
文献类型：
--
作者：
KOHCHI, T;YOSHIDA, T;OHYAMA, K
光系统II和细胞色素b6/f复合体中的4个组分的基因psbB、psbH、petB和petd在地钱叶绿体中聚集并共转录。在PSBB和PSBH基因间隔区的相对DNA链上，推导出一个由43个有义密码子组成的开放阅读框，命名为ORF43基因。ORF43基因在地钱叶绿体中转录活跃。PSBB操纵子的ORF43转录本。异源Northern杂交结果表明，ORF43基因在豌豆幼苗中的转录水平随着绿色化程度的增加而增加。这是在叶绿体中首次发现差异重叠转录。
The genes psbB, psbH, petB and petD for the components in photosystem II and the cytochrome b6/f complex are clustered and co-transcribed in liverwort Marchantia polymorpha chloroplasts. On the opposite DNA strand in spacer region between the genes psbB and psbH, we deduced an open reading frame consisting of 43 sense codons, and designated it as the ORF43 gene. The ORF43 gene was actively transcribed in liverwort chloroplasts. The ORF43 transcripts of the psbB operon. Heterogeneous Northern hybridization showed that the mRNA transcripts for the ORF43 gene increased with greening in pea seedlings. This is the first demonstration of divergent overlapping transcription in chloroplasts.