Cholesterol Increases Ventricular Volume in a Rabbit Model of Alzheimer's Disease
Cholesterol Increases Ventricular Volume in a Rabbit Model of Alzheimer's Disease
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DOI：
10.3233/jad-2011-111415
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发表时间：
2012-01-01
影响因子：
4
通讯作者：
Schreurs, Bernard G.
中科院分区：
文献类型：
--
作者：
Deci, Stephen;Lemieux, Susan K.;Schreurs, Bernard G.
阿尔茨海默病的特征之一是心室容积显著增加。到目前为止，我们和其他人已经表明，胆固醇喂养的阿尔茨海默病兔模型显示多达14种不同的阿尔茨海默病病理标志物，包括淀粉样蛋白-β积累、硫磺素-S染色、血脑屏障破坏、小胶质细胞活化、脑血管系统变化以及学习和记忆的改变。使用3 T的结构磁共振成像，我们现在报告说，胆固醇喂养的兔子在10周后，心室容积也显着增加2%的胆固醇饮食。体积的增加在很大程度上是由于第三脑室的大小增加。这些变化伴随着皮质和海马中β淀粉样蛋白免疫阳性细胞数量的显著增加。在饮用水中添加0.24 ppm铜时，皮质中淀粉样β神经元的数量也会增加。连同其他病理标志物的列表，目前的结果进一步增加了胆固醇喂养的兔子作为阿尔茨海默病的非转基因动物模型的价值的有效性。
One of the hallmarks of Alzheimer's disease is a significant increase in ventricular volume. To date we and others have shown that a cholesterol-fed rabbit model of Alzheimer's disease displays as many as fourteen different pathological markers of Alzheimer's disease including amyloid-beta accumulation, thioflavin-S staining, blood brain barrier breach, microglia activation, cerebrovasculature changes, and alterations in learning and memory. Using structural magnetic resonance imaging at 3T, we now report that cholesterol-fed rabbits also show a significant increase in ventricular volume following 10 weeks on a diet of 2% cholesterol. The increase in volume is attributable in large part to increases in the size of the third ventricle. These changes are accompanied by significant increases in the number of amyloid-beta immuno-positive cells in the cortex and hippocampus. Increases in the number of amyloid-beta neurons in the cortex also occurred with the addition of 0.24 ppm copper to the drinking water. Together with a list of other pathological markers, the current results add further validity to the value of the cholesterol-fed rabbit as a non-transgenic animal model of Alzheimer's disease.