Functional association between female sperm storage organs and male sperm removal organs in calopterygid damselflies
Functional association between female sperm storage organs and male sperm removal organs in calopterygid damselflies
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DOI：
10.1111/j.1479-8298.2005.00123.x
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发表时间：
2005-09-01
影响因子：
0.9
通讯作者：
Tsuchiya, K
中科院分区：
文献类型：
--
作者：
Hayashi, F;Tsuchiya, K
蝶蝶科的雌性豆娘有两个储存精子的器官：一个球形的囊交配基质和一个管状的精囊。雄性苍蝇有一种特殊的喙，它有一个弯曲的头，用来去除法囊精子，它有侧面的刺突，用来去除精囊精子。不同物种和种群的侧突相对于精囊管的宽度不同：较窄的侧突在物理上能够接触到精囊精子，而较宽的侧突则不能。在本研究中，采用一种基于活/死双荧光的新方法，比较了两种具有不同精子结构的Calopteryx cornelia和Mnais pruinosa的精子储存模式和精子结构，不仅在精子数量（数量）方面，而且在精子质量（活力）方面。Calopteryx cornelia是一种典型的精子清除器。在这个物种中，法囊精子和精囊精子的生存能力相似。相比之下，在M. pruinosa中，精囊比囊小得多，通常不含精子。即使这个物种的精子囊确实含有精子，也有很高比例的精子已经死亡。尽管M. pruinosa的精囊具有这样的萎缩倾向，但雄性的精囊仍发育出类似于C. cornelia的长而多刺的侧突，这表明该突除除精囊外还有其他功能。它们可能起着阻止或引导的作用，以操纵喙状头部从滑囊中取出精子团。
Female damselflies in the family Calopterygidae have two sperm storage organs: a spherical bursa copulatrix and a tubular spermatheca. Male flies have a peculiar aedeagus with a recurved head with which to remove bursal sperm, and lateral spiny processes to remove spermathecal sperm. The lateral processes differ among species and populations in terms of their width relative to the spermathecal duct: the narrower processes are physically able to access spermathecal sperm, while the wider ones are not. In the present study, sperm storage patterns and aedeagal structures were compared between two calopterygid species with different spermathecal structures - Calopteryx cornelia and Mnais pruinosa - with respect to not only sperm quantity (number) but also sperm quality (viability), by using a recently developed method based on live/dead dual fluorescence. Calopteryx cornelia is a typical spermathecal sperm remover. In this species, viability was similar between bursal and spermathecal sperm. In contrast, in M. pruinosa, the spermatheca was much smaller than the bursa and often contained no sperm. Even when the spermatheca of this species did contain sperm, a high percentage of it was dead. Although the spermatheca of M. pruinosa has such atrophic tendencies, males have nevertheless developed long and spiny lateral processes similar to those of C. cornelia, suggesting the processes have functions other than spermathecal sperm removal. They possibly function as stoppers or guides for manipulating the aedeagal head to remove the sperm mass from the bursa.