Viscoelastic properties of leukocytes.
Viscoelastic properties of leukocytes.
复制标题
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
S. Chien;G. Schmid-Schönbein;Sung Kl;Schmalzer Ea;R. Skalak
中科院分区：
文献类型：
--
作者：
S. Chien;G. Schmid-Schönbein;Sung Kl;Schmalzer Ea;R. Skalak
小变形期间白细胞（WBC）的粘弹性特性通过微量移液器抽吸测定。悬浮在无 Ca2+ 介质中的中性粒细胞响应步进抽吸压力的被动变形行为包括最初的快速弹性响应，随后是蠕变位移。这些与时间相关的响应可以通过粘弹性固体进行建模，其中弹性元件 (K1) 与麦克斯韦元件并联，麦克斯韦元件由另一个弹性元件 (K2) 与粘性元件 (mu) 串联组成。温度变化（9-40 摄氏度）会导致 mu 发生反向变化，但对 K1 和 K2 没有影响。所有三个系数均不受 pH 值降至 5.4 的影响；然而，当pH大于或等于7.8时，K1和mu增加。渗透压的增加会导致所有三个系数增加，尤其是 mu。秋水仙碱处理导致 mu 和 K2 选择性降低，而不影响 K1。 B 淋巴细胞的粘弹性系数与中性粒细胞相似，但 T 淋巴细胞的这些系数值更高。在 2 mM Ca2+ 存在下，中性粒细胞比不含 Ca2+ 的培养基具有更高的粘弹性系数，并且它们形成比主细胞体对微量移液器抽吸变形具有更大抵抗力的原足。形态分析表明，由于膜折叠的存在，白细胞具有较大的多余膜面积，这有利于白细胞在恒定面积下变形。在通过 5 微米筛子过滤时，白细胞比红细胞更容易堵塞孔道，因为它们的细胞粘度较高且存在细胞核。白细胞的流变特性对其在微循环中的功能和流动动力学具有重要影响。
The viscoelastic properties of leukocytes (WBCs) during small deformation were determined by micropipette aspiration. The passive deformation behavior of neutrophils suspended in a Ca2+-free medium in response to a step aspiration pressure consists of an initial rapid, elastic response followed by a creep displacement. These time-dependent responses can be modeled by a viscoelastic solid in which an elastic element (K1) is in parallel with a Maxwell element composed of another elastic element (K2) in series with a viscous element (mu). Variations in temperature (9-40 degrees C) cause an inverse change in mu, but have no effects on K1 and K2. All three coefficients are not affected by decreases in pH down to 5.4; with pH greater than or equal to 7.8, however, K1 and mu increase. Increases in osmolality cause a rise in all three coefficients, especially mu. Colchicine treatment results in selective decreases in mu and K2 without affecting K1. B lymphocytes have viscoelastic coefficients similar to those of neutrophils, but T lymphocytes have higher values for these coefficients. In the presence of 2 mM Ca2+, the neutrophils have higher viscoelastic coefficients than in Ca2+-free medium, and they form protopods which have greater resistance than the main cell body to deformation by micropipette aspiration. Morphometric analysis shows that WBCs have large excess membrane area due to the presence of membrane foldings, which facilitate WBC deformation at constant area. During filtration through 5-micrometers sieves, WBCs are much more prone to pore plugging than erythrocytes because of their higher cellular viscosity and the presence of nucleus. The rheological properties of WBCs have significant implications in their functions and flow dynamics in the microcirculation.