Fine structure of clonally propagated in vitro life stages of a Perkinsus sp isolated from the baltic clam Macoma balthica
Fine structure of clonally propagated in vitro life stages of a Perkinsus sp isolated from the baltic clam Macoma balthica
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DOI：
10.1111/j.1550-7408.2001.tb00414.x
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发表时间：
2001-01-01
影响因子：
2.2
通讯作者：
Vasta, GR
中科院分区：
文献类型：
--
作者：
Coss, CA;Robledo, JAF;Vasta, GR
我们建立了 Perkinsus sp 的单克隆体外培养物。从波罗的海蛤 Macoma balthica 中分离出来，并通过光学和透射电子显微镜将不同生命阶段的形态特征与目前公认的 Perkinsus 物种：Perkinsus marinus、Perkinsus olseni 进行比较。大西洋帕金苏斯和库格瓦迪帕金苏斯。除了滋养体比 P. marinus 稍大，并且它们在培养物中经历游动孢子形成之外，在光学显微镜下观察我们的分离物并没有发现与任何 Perkinsus 物种的显着差异。帕金瑟斯 sp。 M. balthica 与其他 Perkinsus 物种具有良好的结构特征，这显然将其归入该属。虽然 Perkinsus sp 的游动孢子。 M. balthica 的体形略小于其他物种，超微结构的排列以及上皮复合体和鞭毛的外观似乎与 P. marinus 和 P. atlanticus 相同。在某些部分，我们的分离株还出现了除前端和分叶状线粒体以外的区域的质膜皮质肺泡扩张，据报道，这些区域是 P. qugwadi 所独有的。在将分类学权重分配给任何特定形态特征以指定 Perkinsus 物种方面，作者之间几乎没有达成共识。目前对总体形态和超微结构的研究得到了其他地方报道的分子研究的补充，这些研究表明 Perkinsus sp。来自Macoma balthica 的Macoma balthica 是一个独特的物种。
We established monoclonal in vitro cultures of a Perkinsus sp. isolated from the baltic clam Macoma balthica and compared morphological features of various life stages by light and transmission electron microscopy to those of the currently accepted Perkinsus species: Perkinsus marinus, Perkinsus olseni. Perkinsus atlanticus, and Perkinsus qugwadi. Except that trophozoites were slightly larger than those of P. marinus, and that they underwent zoosporulation in culture, observation of our isolate under light microscopy did not reveal striking differences from any Perkinsus species. Perkinsus sp. from M. balthica shared fine structural characteristics with other Perkinsus species that clearly place it within this genus. Although zoospores of Perkinsus sp. from M. balthica were slightly smaller than those from other species, the ultrastructural arrangement and appearance of the epical complex and flagella seem to be identical to those of P. marinus and P. atlanticus. Our isolate also appeared, in some sections, to have cortical alveolar expansions of the plasmalemma at regions other than the anterior end and lobulated mitochondria that were reported as unique for P. qugwadi Little consensus exists among authors in the assignment of taxonomic weight to any particular morphological feature to designate Perkinsus species. The present study of gross morphology and ultrastructure was complemented with molecular studies reported elsewhere, which propose that Perkinsus sp. from Macoma balthica is a distinct species.