Monoclonal antibodies as probes of acetylcholine receptor structure. 1. Peptide mapping.
Monoclonal antibodies as probes of acetylcholine receptor structure. 1. Peptide mapping.
复制标题
单克隆抗体作为乙酰胆碱受体结构的探针。
DOI：
10.1021/bi00511a016
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Lindstrom,J
中科院分区：
文献类型：
--
作者：
Gullick,WJ;Tzartos,S;Lindstrom,J
William J. Gullick、Socrates Tzartos和Jon Lindstrom* 摘要：用蛋白水解酶消化来自加利福尼亚电鳐的乙酰胆碱受体的分离亚基，并通过凝胶电泳分析所得多肽片段。我们已经确定了那些片段，其中含有碳水化合物和那些从α亚基标记的乙酰胆碱结合位点特异性试剂[4-（Ar-maleimido）苄基]三[3 H]甲基碘化铵。我们已经测试了几种针对来自鱼雷的乙酰胆碱受体的单克隆抗体，其中一些与变性亚基反应[Tzartos，S. J.，& Lindstrom，J. M.等人（1980）Proc. Acad. Sci. USA 77，755; Tzartos，S. J.，& Lindstrom，J. M.（1981）在Monclonal Antibodies in Endocrine Research（Fellows，R.，& Eisenbarth，G.，Eds.）Raven Press（in press）]。来自加利福尼亚电鳐（Torpedo californica）的电器官的这些-Axetylcholine受体（AcChR）1的结合特异性由四个糖蛋白亚基组成，比例为α 2 β 8（Reynolds & Karlin，1978; Lindstrom et al.，1979 a; Raftery等人，1980年）。这些亚基的表观分子量取决于所用的电泳系统和标准品，但分别为约40000、50000、57000和64000（Weill等人，1974; Raftery等人，1975; Hucho等人，1978; Chang & Bock，1977; Lindstrom等人，1978，1979a; Froehner & Rafto，1979）。在250000分子量单体内（Martinez-Carrion等人，
William J. Gullick, Socrates Tzartos, and Jon Lindstrom* abstract: The isolated subunits of the acetylocholine receptor from Torpedo californica were digested with proteolytic en-zymes, and the resulting polypeptide fragments were analyzed by gel electrophoresis. We have identified those fragments which contain carbohydrate and those from the a subunit which are labeled with the acetylcholine binding site specific reagent [4-(Ar-maleimido) benzyl] tri [3H] methylammonium iodide. We have tested several monoclonal antibodies raised to the acetylcholine receptor from torpedo, some of which react with the denatured subunits [Tzartos, S. J., & Lindstrom, J. M.(1980) Proc. Natl. Acad. Sci. USA 77, 755; Tzartos, S. J., & Lindstrom, J. M.(1981) in Monclonal Antibodies in Endocrine Research (Fellows, R., & Eisenbarth, G., Eds.) Raven Press (in press)]. The binding specificities of these-Axetylcholine receptor (AcChR) 1 from the electric organ of Torpedo californica consists of fourglycoprotein subunits in the ratio a2Py8 (Reynolds & Karlin, 1978; Lindstrom et al., 1979a; Raftery et al., 1980). The apparent molecular weights of these subunits depend on the electrophoresis system and standards employed but are about 40000, 50000, 57 000, and 64000, respectively (Weill et al., 1974; Raftery et al., 1975; Hucho et al., 1978; Chang & Bock, 1977; Lindstrom et al., 1978, 1979a; Froehner & Rafto, 1979). Within the 250000 molecular weight monomer(Martinez-Carrion et al.,