Phorbol myristate acetate stimulates microtubule and 10-nm filament extension and lysosome redistribution in mouse macrophages.
Phorbol myristate acetate stimulates microtubule and 10-nm filament extension and lysosome redistribution in mouse macrophages.
复制标题
植物肉芽菌醋酸盐刺激小鼠巨噬细胞中的微管和10 nm细丝延伸和溶酶体重新分布。
DOI：
10.1083/jcb.86.2.641
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发表时间：
1980-08
影响因子：
7.8
通讯作者：
Wang, E
中科院分区：
文献类型：
--
作者：
Phaire-Washington, L;Silverstein, S C;Wang, E
肉豆蔻酸酯Phorbol acetate （PMA）刺激小鼠腹膜巨噬细胞的细胞扩散和液相胞饮。秋水仙碱（10(-5)M）和细胞松弛素B （10(-5) M）消除PMA刺激的胞饮作用，但对细胞扩散影响不大（Phaire-Washington等，1980，J. Cell Biol.）。, 86:634 - 640)。我们在这里报告PMA也改变细胞骨架的组织和细胞器在这些细胞中的分布。对照组和pma处理的巨噬细胞都不含肌动蛋白电缆。经pma处理的巯基乙酸引发的巨噬细胞在其底物粘附膜下表现出许多随机分布的点状灶，这些点状灶明亮地染色为肌动蛋白。秋水仙碱（10(-5)M）或细胞松弛素B （10(-5) M）不会改变这些含有肌动蛋白的灶的外观和分布。在巯基乙酸诱导的巨噬细胞中，PMA引起微管和10nm丝的延伸和径向组织，并促进次级溶酶体从核周位置向外周细胞质移动。根据所使用的PMA浓度，45-71%的巯基乙酸诱导的巨噬细胞和32-44%的蛋白酶-蛋白胨诱导的巨噬细胞和大量溶酶体从中心球区辐射，沿微管和10nm丝束呈线性排列。秋水仙碱（10(-5)M）和鬼臼毒素（10(-5)M）阻止微管、10-nm细丝和溶酶体在这些细胞中的径向重新分布。细胞松弛素B和D （10(-5) M）对这些过程没有抑制作用。这些发现表明，微管和10nm细丝以协调的方式响应PMA和抑制微管功能的药物；他们认为这些细胞骨架元件调节巨噬细胞细胞质中溶酶体的运动和分布。
Phorbol myristate acetate (PMA) stimulates cell spreading and fluid- phase pinocytosis in mouse peritoneal macrophages. Colchicine (10(-5) M) and cytochalasin B (10(-5) M) abolish PMA stimulated pinocytosis but have little effect on cellular spreading (Phaire-Washington et al., 1980, J. Cell Biol., 86:634-640). We report here that PMA also alters the organization of the cytoskeleton and the distrubution of organelles in these cells. Neither control nor PMA-treated macrophages contain actin cables. PMA-treated resident thioglycolate-elicited macrophages exhibit beneath their substrate-adherent membranes many randomly distributed punctate foci that stain brightly for actin. The appearance and distribution of these actin-containing foci are not altered by colchicine (10(-5) M) or cytochalasin B (10(-5) M). In thioglycolate- elicited macrophages PMA causes the extension and radial organization of microtubules and 10-nm filaments and promotes the movement of secondary lysosomes from their perinuclear location to the peripheral cytoplasm. Depending upon the concentration of PMA used, 45-71% of thioglycolate-elicited macrophages and 32-44% of proteose-peptone- elicited macrophages and numerous lysosomes, radiating from the centrosphere region, arranged linearly along microtubule and 10-nm filament bundles. Colchicine (10(-5) M) and podophyllotoxin (10(-5) M) prevent the radial redistribution of microtubules, 10-nm filaments, and lysosomes in these cells. Cytochalasins B and D (10(-5) M) have no inhibitory effects on these processes. These findings indicate that microtubules and 10-nm filaments respond in a coordinated fashion to PMA and to agents that inhibit microtubule function; they suggest that these cytoskeletal elements regulate the movement and distribution of lysosomes in the macrophage cytoplasm.