Properties and activity of the lipopolysaccharide-receptor from human erythrocytes.
Properties and activity of the lipopolysaccharide-receptor from human erythrocytes.
复制标题
人红细胞脂多糖受体的特性和活性。
DOI：
10.1021/bi00704a011
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
B. Jirgensons
中科院分区：
文献类型：
--
作者：
G. Springer;J. Adye;A. Bezkorovainy;B. Jirgensons
george F.施普林格，* James C. Adye， Anatoly Bezkorovainy和Bruno Jirgensons摘要：我们从人红细胞膜中分离出一种理化均相的脂蛋白，分子量为256,000。它富含v -乙酰神经氨酸、半乳糖和己糖胺。完整的物质阻止了所有革兰氏阴性细菌未加热和加热、光滑和粗糙的脂多糖和蛋白脂多糖对红细胞的附着。它不与其他细菌抗原相互作用，因此被称为“脂多糖受体”。该受体物理可逆地阻断了附着在红细胞上的脂多糖。在标准条件下，citraconylation和dissociating聚丙烯酰胺凝胶电泳均产生1个大片段和1个小片段。
Georg F. Springer,* James C. Adye, Anatoly Bezkorovainy, and Bruno Jirgensons abstract: We have isolated from human erythrocyte mem-branes a physicochemically homogeneous lipoglycoprotein with a molecular weight of 256,000. It is rich in V-acetylneuraminic acid, galactose, and hexosamines. The intact substance prevented attachment to erythrocytes of unheated and heated, smooth and rough lipopolysaccharide and protein-lipopolysaccharide of all gram-negative bacteriatested. It did not interact with other bacterial antigens and therefore is referred to as “lipopolysaccharide-receptor.” The receptor physically and reversibly blocked those groupings on lipo-polysaccharide which attach to red cells. Both citraconylation and dissociating polyacrylamide gel electrophoresis under standard conditions produced 1 large and 1 small fragment.