The light-up fluorescence of AgNCs in a "DNA bulb"
The light-up fluorescence of AgNCs in a "DNA bulb"
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“DNA 灯泡”中 AgNC 的发光荧光
DOI：
10.1039/c8nr02575d
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发表时间：
2018
期刊：
影响因子：
6.7
通讯作者：
Pei Renjun
中科院分区：
文献类型：
--
作者：
Guo Yahui;Shen Fumiao;Cheng Yuliang;Xie Yunfei;Yu Hang;Yao Weirong;Li Hung-Wing;Qian He;Pei Renjun
在这项工作中，我们构建了一系列设计和实验来研究 DNA 结构如何影响银纳米簇 (AgNC) 的荧光。首先以“球状”DNA结构为合成模板，得到了发出亮绿光的AgNCs，量子产率为17.2%。 “DNA球”结构是使用三个单链寡核苷酸（ssDNA）组装而成，其中灵活的ssDNA部分充当“细丝”。结果似乎很有趣，因为只有形成完整的空间结构才能获得发光的 AgNC。为了进一步研究“球茎”结构的作用，分别采用十三个富含胞嘧啶的序列作为“丝”。有趣的是，当“丝状”ssDNA 整合到“球状”结构中后，AgNC 的荧光强度均得到增强。增强幅度高达175.2倍，最小增强幅度为1.3倍。然后进行光谱分析以了解胞嘧啶的作用。当“球壳”中的序列发生变化时，“DNA球”结构仍然表现出增强作用。最后，发射绿光的 AgNC 用于选择性检测 Hg2+，以展示潜在的应用。
In this presented work, a series of designs and experiments have been constructed to study how a DNA structure affected the fluorescence of silver nanoclusters (AgNCs). A “bulb”-like DNA structure was firstly used as the synthesizing template, and bright green-emitting AgNCs were obtained with a quantum yield of 17.2%. The “DNA bulb” structure was assembled using three single-stranded oligonucleotides (ssDNAs), with a flexible ssDNA part serving as the “filament”. The results seemed intriguing as luminous AgNCs could be obtained only when the complete spatial structure was formed. To further investigate the role of the “bulb” structure, thirteen cytosine-rich sequences were employed as the “filament”, respectively. Interestingly, the fluorescence intensities of AgNCs were all enhanced after the “filament” ssDNA was integrated into the “bulb” structure. The enhancement was as much as 175.2-fold, and the minimum enhancement was 1.3-fold. Spectral analyses were then conducted to give an understanding of the role of cytosine. When the sequences in the “bulb shell” were changed, the “DNA bulb” structure still showed an enhancing effect. Finally, the green-emitting AgNCs were utilized toward the selective detection of Hg2+ to demonstrate potential applications.