DURATION OF CYCLE OF SEMINIFEROUS EPITHELIUM AND SPERMATOGONIAL RENEWAL IN MONKEY MACACA-ARCTOIDES
DURATION OF CYCLE OF SEMINIFEROUS EPITHELIUM AND SPERMATOGONIAL RENEWAL IN MONKEY MACACA-ARCTOIDES
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DOI：
10.1002/aja.1001360204
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发表时间：
1973-01-01
影响因子：
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通讯作者：
ANTAR, M
中科院分区：
文献类型：
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作者：
CLERMONT, Y;ANTAR, M
在腹膜内注射3 H-胸苷后3小时和12天-3小时处死猴。制备睾丸高碘酸-希夫-苏木精染色切片的放射自显影照片。在3小时时，进化最多的标记生殖细胞是周期第VIII阶段的细线期精母细胞;在12天-3小时时，进化最多的标记细胞是下一周期第IX阶段的粗线期精母细胞。因此，在12天的时间间隔内，最进化的标记精母细胞已经通过略多于一个完整的周期。含有标记精母细胞的管状横截面的定量分析允许计算生精上皮的一个周期的持续时间，结果是11.6天。精子发生的整个过程，从第一次精原细胞有丝分裂发生在周期的第八阶段开始，到精子释放发生在周期的第六阶段结束，在相同的组织学材料上，在周期的不同阶段对静止和分裂的精原细胞进行计数，以确定它们的模式。增殖和更新。6个有丝分裂高峰分别位于周期的第VIII、X、XII、II、IV和VI期。细胞计数和标记指数表明，苍白A型精原细胞是在6个分裂峰的前2个峰分裂的细胞，而B型精原细胞是在其余4个分裂峰分裂的细胞。暗A型精原细胞在周期中未见分裂，可能被认为是“储备干细胞”。细胞计数和细胞比率进一步表明，苍白A型精原细胞在周期的第VIII期分裂（和一些在第IX阶段）产生大约两倍数量的苍白A型精原细胞：在几种可能的方案中，作者认为，这些中的一半进入一个长的间期，并成为在下一个周期中形成精原细胞的干细胞;这些可能被称为“更新干细胞”;我们认为，有丝分裂第VIII期高峰期产生的苍白A型精原细胞的另一半在第X期分裂产生B型精原细胞。然后，后者的细胞可以进入一系列的四个连续的有丝分裂，在此期间，他们的数量增加一倍，每次产生一代初级精母细胞。
Monkeys were sacrificed at three hours and at 12 days‐3 hours after an intraperitoneal injection with3H‐thymidine. Radioautographs were prepared of periodic acid‐Schiff‐hematoxylin‐stained sections of the testes. At three hours, the most evolved labeled germ cells were leptotene spermatocytes in stage VIII of the cycle; at 12 days‐three hours the most evolved labeled cells were pachytene spermatocytes in stage IX of the succeeding cycle. Thus, over a 12‐day interval, the most evolved labeled spermatocytes had advanced through slightly more than one complete cycle. A quantitative analysis of tubular cross sections containing labeled spermatocytes permitted the calculation of the duration of one cycle of the seminiferous epithelium which turned out to be 11.6 days. The whole process of spermatogenesis which begins with the first spermatogonial mitoses taking place in stage VIII and terminates with the release of spermatozoa taking place in stage VI of the cycle, extends over the duration of 3.8 consecutive cycles and therefore requires approximately 44 days.On the same histological material counts of resting and dividing spermatogonia were performed at various stages of the cycle to determine their mode of proliferation and renewal. Six mitotic peaks were disclosed and were located in stages VIII, X, XII, II, IV and VI of the cycle. Cell counts and labeling indices indicated that the pale type A spermatogonia were the cells dividing during the first two of these six peaks of mitoses while the type B spermatogonia divided during the remaining four peaks of mitoses. The dark type A spermatogonia were not seen to divide during the cycle and may be considered as “reserve stem cells.” Cell counts and cell ratios indicated further that the pale type A spermatogonia divided in stage VIII of the cycle (and some in stage IX) to yield about twice their number of pale type A spermatogonia: of several possible schemes the authors believe that half of these entered a long interphase and became stem cells for the spermatogonia to be formed during the next cycle; these may be referred to as “renewing stem cells”; we consider that the other half of the pale type A spermatogonia arising from stage VIII peak of mitoses divided in stage X to produce type B spermatogonia. The latter cells then could enter the series of four consecutive mitoses during which they doubled their number each time to yield a generation of primary spermatocytes.