Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas fluorescens
Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas fluorescens
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DOI：
10.1128/jb.180.7.1939-1943.1998
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发表时间：
1998-04-01
影响因子：
3.2
通讯作者：
Ligon, JM
中科院分区：
文献类型：
--
作者：
Kirner, S;Hammer, PE;Ligon, JM
吡咯硝酸酯是色氨酸的次生代谢物，具有较强的抗真菌活性。最近，我们从荧光假单胞菌中描述了四个编码吡咯硝酸酯生物合成的基因prnABCD。在这里介绍的工作中，我们描述了每个prn基因产物的功能。这四个基因编码的蛋白质在大小和血清学上与野生型荧光假单胞菌中的蛋白质相同，但没有一个突变体，即整个prn基因区域已被删除。PRNA基因产物催化L-色氨酸氯化生成7-氯-L-色氨酸。PrnB基因产物催化环重排和脱羧基反应，将7-氯-L-色氨酸转化为一氯氨基吡咯硝酸酯。PrNC基因产物在3位氯化一氯氨基吡咯硝酸酯，生成氨基吡咯硝酸酯。PrnD基因产物催化氨基吡咯硝酸氨基氧化成硝基，形成吡咯硝酮。操纵子中prn基因的组织与生物合成途径中的反应顺序相同。
Pyrrolnitrin is a secondary metabolite derived from tryptophan and has strong antifungal activity. Recently we described four genes, prnABCD, from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. In the work presented here, we describe the function of each prn gene product. The four genes encode proteins identical in size and serology to proteins present in wild-type Pseudomonas fluorescens, but absent from a mutant from which the entire prn gene region had been deleted. The prnA gene product catalyzes the chlorination of L-tryptophan to form 7-chloro-L-tryptophan. The prnB gene product catalyzes a ring rearrangement and decarboxylation to convert 7-chloro-L-tryptophan to monodechloroaminopyrrolnitrin. The prnC gene product chlorinates monodechloroaminopyrrolnitrin at the 3 position to form aminopyrrolnitrin. The prnD gene product catalyzes the oxidation of the amino group of aminopyrrolnitrin to a nitro group to form pyrrolnitrin. The organization of the prn genes in the operon is identical to the order of the reactions in the biosynthetic pathway.