Efficient production of a human tumour necrosis factor in Escherichia coli.
Efficient production of a human tumour necrosis factor in Escherichia coli.
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在大肠杆菌中高效生产人类肿瘤坏死因子。
DOI：
10.1016/0168-1656(85)90015-x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
S. Nakamura
中科院分区：
文献类型：
--
作者：
T. Fukui;J. Yamagishi;Y. Furutani;M. Notake;M. Yamayoshi;H. Nomura;M. Yamada;S. Nakamura
以人肺泡巨噬细胞肿瘤坏死因子(TNF)基因为模板，从人肺泡巨噬细胞肿瘤坏死因子(TNF)基因文库中克隆了人肿瘤坏死因子(TNF)基因。利用该基因构建了多种表达载体，并对其在大肠杆菌中的表达能力进行了检测。最好的转化子产生了一种肿瘤坏死因子多肽，产量约为细胞蛋白的20%。纯化的肿瘤坏死因子多肽在N-端没有蛋氨酸残基，具有预期的理化性质和肿瘤坏死因子的生物活性特征。
Human tumour necrosis factor (TNF) cDNA was isolated from a cDNA library prepared using TNF mRNA from human alveolar macrophages. Using the cDNA, various expression plasmids were constructed and their TNF producibility was examined inEscherichia coli. The best transformants produced a TNF polypeptide in a yield of about 20% of cellular proteins. The purified TNF polypeptide was lacking a methionine residue at the N-terminus, and possessed the expected physical and chemical properties and the biological activities characteristic of TNF.