Electrochemiluminescence of Supramolecular Nanorods and Their Application in the "On-Off-On" Detection of Copper Ions.
Electrochemiluminescence of Supramolecular Nanorods and Their Application in the "On-Off-On" Detection of Copper Ions.
复制标题
DOI：
10.1002/chem.201504995
复制
发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Yan-Mei Lei;Min Zhao;Ai Wang;Yanqing Yu;Y. Chai;R. Yuan;Ying Zhuo
中科院分区：
文献类型：
--
作者：
Yan-Mei Lei;Min Zhao;Ai Wang;Yanqing Yu;Y. Chai;R. Yuan;Ying Zhuo
本工作利用3，4，9，10-二萘嵌苯四甲酸（PTCA）与苯胺的超分子相互作用，构建了一种新型的电化学发光（ECL）超分子纳米棒发光体，并将其应用于铜离子（Cu（2+））检测的电化学发光生物传感器中，实现了“on-off-on”开关系统。通过使用超分子纳米棒与一种新的信号放大策略，包括共反应加速器，获得了具有强而稳定的ECL发射的初始“信号开启”状态。此外，通过将氨基二茂铁（Fc-NH 2）固定在铜基链修饰的Au纳米粒子上，制备了电化学发光猝灭探针（Fc-NH 2/Cu-Sub/nano-Au）。猝灭剂探针与固定化的Cu-酶链杂交形成Cu（2+）特异性DNA酶。类似地，通过Fc-NH 2对激发态PTCA的ECL的高猝灭效应获得“信号关闭”状态（（1）PTCA*）。正如预期的那样，第二个“开启”状态可以通过与靶Cu（2+）孵育来实现，这是由于Cu（2+）特异性DNA酶被不可逆地切割，导致猝灭剂探针从传感器界面释放。基于与目标Cu（2+）孵育前后的电化学发光强度变化（ΔIECL），制备的Cu（2+）特异性DNA酶生物传感器用于Cu（2+）浓度的测定，具有高灵敏度、高选择性和良好的再生性。
In this work, an "on-off-on" switch system has been successfully applied through the construction of an electrochemiluminscent biosensor for copper ion (Cu(2+) ) detection based on a new electrochemiluminescence (ECL) emitter of supramolecular nanorods, which was achieved through supramolecular interactions between 3,4,9,10-perylenetetracarboxylic acid (PTCA) and aniline. The initial "signal-on" state with strong and stable ECL emission was obtained by use of the supramolecular nanorods with a new signal amplification strategy involving a co-reaction accelerator. In addition, ECL quencher probes (Fc-NH2 /Cu-Sub/nano-Au) were fabricated by immobilizing aminoferrocene (Fc-NH2 ) on Cu-substrate strand modified Au nanoparticles. The quencher probes were hybridized with the immobilized Cu-enzyme strand to form Cu(2+) -specific DNAzyme. Similarly, the "signal-off" state was obtained by the high quenching effect of Fc-NH2 on the ECL of the excited-state PTCA ((1) PTCA*). As expected, the second "switch-on" state could achieved by incubating with the target Cu(2+) , owing to the Cu(2+) -specific DNAzyme, which was irreversibly cleaved, resulting in the release of the quencher probes from the sensor interface. Herein, on the basis of the ECL intensity changes (ΔIECL ) before and after incubating with the target Cu(2+) , the prepared Cu(2+) -specific DNAzyme-based biosensor was used for the determination of Cu(2+) concentrations with high sensitivity, excellent selectivity, and good regeneration.