Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass.

Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass.
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使用 DLC 涂层玻璃上的 DNA 微阵列识别 SV-T2 细胞上的候选细胞表面标记。

DOI:
10.1016/j.bbrc.2005.06.083
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发表时间:
2005
影响因子:
3.1
通讯作者:
M. Seno
M. Seno
中科院分区:
生物学4区
文献类型:
--
作者:
Tuoya;K. Hirayama;Tadahiro Nagaoka;Dongwei Yu;Takayuki Fukuda;H. Tada;Hidenori Yamada;M. Seno

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We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
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