Heterogeneous expressions of hepcidin isoforms in hepatome-derived cells detected using simultaneous LC-MS/MS
Heterogeneous expressions of hepcidin isoforms in hepatome-derived cells detected using simultaneous LC-MS/MS
复制标题
使用同步 LC-MS/MS 检测肝组来源细胞中铁调素亚型的异质表达
DOI：
10.1002/prca.200900112
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发表时间：
2009
期刊：
影响因子：
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et al
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文献类型：
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作者：
Hosoki T;Ikuta K;et al
Hepcidin是铁稳态的关键调节因子，已知有三种亚型：Hepcidin - 20、- 22和- 25。Hepcidin - 25被认为是主要的亚型，也是唯一已知参与铁代谢的亚型；其他同工异构体的生理作用尚不清楚。由于其参与遗传性血色素沉着病和慢性病贫血的病理生理，hepcidin表达的调控机制已被广泛研究，但大多数研究仅在转录水平上进行。hepcidin的检测困难阻碍了体外研究。在本研究中，我们开发了一种同时定量肝癌来源细胞系培养基中hepcidin‐20、‐22和‐25的新方法。利用这种方法，我们确定了hepcidin亚型的表达模式以及人肝癌来源的培养细胞对各种刺激的反应模式。我们发现细胞系之间存在巨大差异。总之，提出了一种同时测定hepcidin亚型的新方法。该方法揭示了hepcidin亚型在人肝癌来源细胞中的异质表达。我们相信我们的方法将有助于定量研究hepcidin在铁稳态中的作用。
Hepcidin, a key regulator of iron homeostasis, is known to have three isoforms: hepcidin‐20, ‐22, and ‐25. Hepcidin‐25 is thought to be the major isoform and the only one known to be involved in iron metabolism; the physiological roles of other isoforms are poorly understood. Because of its involvement in the pathophysiology of hereditary hemochromatosis and the anemia of chronic disease, the regulatory mechanisms of hepcidin expression have been extensively investigated, but most studies have been performed only at the transcriptional level. Difficulty in detecting hepcidin has impededin vitroresearch. In the present study, we developed a novel method for simultaneous quantification of hepcidin‐20, ‐22, and ‐25 in the media from hepatoma‐derived cell lines. Using this method, we determined the expression patterns of hepcidin isoforms and the patterns of responses to various stimuli in human hepatoma‐derived cultured cells. We found substantial differences among cell lines. In conclusion, a novel method for simultaneous quantification of hepcidin isoforms is presented. Heterogeneous expressions of hepcidin isoforms in human hepatoma‐derived cells were revealed by this method. We believe our method will facilitate quantitative investigation of the role hepcidin plays in iron homeostasis.