Heterogeneity and temporal dynamics of evolution of G1 human rotaviruses in a settled population
Heterogeneity and temporal dynamics of evolution of G1 human rotaviruses in a settled population
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DOI：
10.1128/jvi.00340-06
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Martella, Vito
中科院分区：
文献类型：
--
作者：
Arista, Serenella;Giammanco, Giovanni M.;Martella, Vito
从连续19年收集的轮状病毒样本被用来调查的异质性和G1轮状病毒株在地理上定义的人口演变的动态。对G1 P [8]人轮状病毒株VP 7基因序列的系统发育分析显示了一个由三个谱系和七个亚谱系组成的异质群体的循环。G1轮状病毒传播的增加显然与新型G1毒株的引入有关，与前几年传播的毒株相比，新型G1毒株在参与轮状病毒中和的抗原区域中表现出多个氨基酸变化。G1抗原变异体的出现和/或引入可能是G1轮状病毒在当地人群中持续循环的原因，在免疫压力机制的影响下，各种谱系和亚谱系以交替方式出现、消失或共循环。对G1株的VP 4编码基因的序列分析显示，较老的毒株与独特的VP 4谱系相关，而1995年后出现了新的VP 4谱系。人轮状病毒疫苗的引入可能会改变驱动轮状病毒进化的力量和平衡，并决定抗原性不同于疫苗制剂中包含的新毒株的传播。在设计疫苗接种策略时，应考虑到人类轮状病毒株中不断出现的VP 7-VP 4基因组合。
A rotavirus sample collection from 19 consecutive years was used to investigate the heterogeneity and the dynamics of evolution of G1 rotavirus strains in a geographically defined population. Phylogenetic analysis of the VP7 gene sequences of G1P[8] human rotavirus strains showed the circulation of a heterogeneous population comprising three lineages and seven sublineages. Increases in the circulation of G1 rotaviruses were apparently associated with the introduction of novel G1 strains that exhibited multiple amino acid changes in antigenic regions involved in rotavirus neutralization compared to the strains circulating in the previous years. The emergence and/or introduction of G1 antigenic variants might be responsible for the continuous circulation of G1 rotaviruses in the local population, with the various lineages and sublineages appearing, disappearing, or cocirculating in an alternate fashion under the influence of immune-pressure mechanisms. Sequence analysis of VP4-encoding genes of the G1 strains revealed that the older strains were associated with a unique VP4 lineage, while a novel VP4 lineage emerged after 1995. The introduction of human rotavirus vaccines might alter the forces and balances that drive rotavirus evolution and determine the spread of novel strains that are antigenically different from those included in the vaccine formulations. The continuous emergence of VP7-VP4 gene combinations in human rotavirus strains should be taken into consideration when devising vaccination strategies.