Synthesis of phosphorylated recognition marker in lysosomal enzymes is located in the cis part of Golgi apparatus.
Synthesis of phosphorylated recognition marker in lysosomal enzymes is located in the cis part of Golgi apparatus.
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溶酶体酶中磷酸化识别标记的合成位于高尔基体的顺式部分。
DOI：
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发表时间：
1982
影响因子：
4.8
通讯作者：
K. von Figura
中科院分区：
文献类型：
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作者：
R. Pohlmann;A. Waheed;A. Hasilik;K. von Figura
大鼠肝膜在蔗糖密度梯度下离心，其中高尔基体被分离成几个亚组分。参与合成溶酶体酶中磷酸化识别标记物的两种酶，udp - n -乙酰氨基葡萄糖胺：溶酶体酶前体n -乙酰氨基葡萄糖-1-磷酸转移酶和α - n -乙酰氨基葡萄糖酰磷酸二酯酶与α -1,2甘露糖苷酶分离，α -1,2甘露糖苷酶是顺式高尔基膜的标记酶，不同于半乳糖基转移酶，反式高尔基膜的标记酶。
Rat liver membranes were subjected to centrifugation in a sucrose density gradient in which the Golgi apparatus was separated into several subfractions. Two enzymes involved in the synthesis of the phosphorylated recognition marker in lysosomal enzymes, UDP-N-acetylglucosamine:lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase and alpha-N-acetylglucosaminyl phosphodiesterase fractionated with alpha-1,2-mannosidase, a marker enzyme of cis Golgi membranes and differently from galactosyltransferase, a marker enzyme of trans Golgi membranes.