Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1.
Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1.
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DOI：
10.1038/ncomms1408
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发表时间：
2011-07-19
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
小泛素相关修饰物(SUMO)是一种调节蛋白质活性、调节植物激素信号、非生物和生物反应的小分子多肽。在这里，我们发现AtSIZ通过其E3相扑连接酶功能来调节拟南芥的氮同化。1-2号花的矮秆植株开花早，种子发育异常，水杨酸含量高，对细菌病原菌的抗性增强。这些突变表型可被外源铵还原为野生型，但不能被硝酸盐、磷酸盐或钾还原。与野生型植株相比，SIZ1-2植株硝酸还原酶活性降低导致氮素浓度低、一氧化氮产量低、硝酸盐含量高。硝酸还原酶NIA1和NIA2被AtSIZ1苏糖化，大大提高了它们的活性。相加和未相加的NiA1和NiA2都可以形成二聚体。我们的结果表明，AtSIZ1通过促进NRs的总甲基化来积极控制拟南芥的氮同化。蛋白质的翻译后修饰是植物对逆境信号的一种反应。在这里，Arabdiposis蛋白SIZ1被证明引起硝酸盐还原酶1和2的SUMO化并增加它们的活性，这表明SIZ1通过SUMO化控制硝酸盐的摄取。
Small ubiquitin-related modifier (SUMO) is a small polypeptide that modulates protein activity and regulates hormone signalling, abiotic and biotic responses in plants. Here we show that AtSIZ regulates nitrogen assimilation in Arabidopsis through its E3 SUMO ligase function. Dwarf plants of siz1-2 flower early, show abnormal seed development and have high salicylic acid content and enhanced resistance to bacterial pathogens. These mutant phenotypes are reverted to wild-type phenotypes by exogenous ammonium but not by nitrate, phosphate or potassium. Decreased nitrate reductase activity in siz1-2 plants resulted in low nitrogen concentrations, low nitric oxide production and high nitrate content in comparison with wild-type plants. The nitrate reductases, NIA1 and NIA2, are sumoylated by AtSIZ1, which dramatically increases their activity. Both sumoylated and non-sumoylated NIA1 and NIA2 can form dimers. Our results indicate that AtSIZ1 positively controls nitrogen assimilation by promoting sumoylation of NRs in Arabidopsis. Posttranslational modification of proteins by small ubiquitin-related modifier is a response to stress signalling in plants. Here, the Arabdiposis protein SIZ1 is shown to cause SUMOylation of nitrate reductases 1 and 2 and to increase their activity, suggesting that SIZ1 controls nitrate uptake via SUMOylation.
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影响因子：
4.3
作者：
Kolbert, Zsuzsanna;Ortega, Leandro;Erdei, Laszlo
通讯作者：
Erdei, Laszlo
影响因子：
7.4
作者：
DEAN, JV;HARPER, JE
通讯作者：
HARPER, JE
DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
影响因子：
21.3
作者：
Dadke, Shrikrishna;Cotteret, Sophie;Chernoff, Jonathan
通讯作者：
Chernoff, Jonathan
影响因子：
4.8
作者：
Goodson, ML;Hong, Y;Sarge, KD
通讯作者：
Sarge, KD