Speciation of Cr (III) and Cr (VI) via Reversed Phase HPLC with Inductively Coupled Plasma Emission Spectroscopic Detection (HPLC-ICP)24
Speciation of Cr (III) and Cr (VI) via Reversed Phase HPLC with Inductively Coupled Plasma Emission Spectroscopic Detection (HPLC-ICP)24
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通过反相 HPLC 和电感耦合等离子体发射光谱检测 (HPLC-ICP) 分析 Cr (III) 和 Cr (VI) 的形态24
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
S. B. Smith
中科院分区：
文献类型：
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作者：
R. Savage;I. Krull;D. Bushee;R. Schleicher;S. B. Smith
用传统的双离子反相高效液相色谱结合折射率(RI)和电感耦合等离子体发射光谱(ICP)检测铬离子，可以可靠、方便、重现性好、快速地分离和检测铬离子，即铬(Cr3=III)和铬酸盐(Cr6=VI)。现在已经开发了许多新的配对离子方法，在流动相中分别使用PICA(四丁基氢氧化铵)或PICB(正烷基磺酸钠)。这允许根据使用的特定离子配对试剂保留每种铬物种，而剩余的铬离子在溶剂前沿洗脱。改变离子配对试剂会颠倒整体情况。每次高效液相分析的总时间约为10分钟。通过两次单独进样，电感耦合等离子体检测提供了一种完整、全面的铬(III)和铬(VI)形态形成方法，并对这两种物种进行了定量。这种使用的方法。
Abstract Chromium ions, viz., chromic (Cr±3=III) and chromate (Cr±6 = VI), can be reliably, conveniently, reproducibly, and quickly separated and detected by the use of conventional paired-ion, reversed phase (RP) high performance liquid chromatography (HPLC) together with refractive index (RI) and/or inductively coupled plasma emission spectroscopic (ICP) detection. A number of novel paired-ion approaches have now been developed, using PIC A (tetrabutylammonium hydroxide) or PIC B (sodium n-alkyl sulfonate) separately in the mobile phase. This allows for the retention of each Cr species depending on the particular ion pairing reagent being used, while the remaining Cr ion elutes in the solvent front. Changing the ion pairing reagent reverses the overall situation. The total time for each HPLC analysis is about 10 mins. ICP detection provides for a complete, overall method of speciation for both Cr (III) and Cr (VI) via two separate injections, together with quantitation for both species. This method of u...