Establishment and characterization of osseous prostate cancer models: Intra tibial injection of human prostate cancer cells
Establishment and characterization of osseous prostate cancer models: Intra tibial injection of human prostate cancer cells
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DOI：
10.1002/pros.10091
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发表时间：
2002-06-01
期刊：
影响因子：
2.8
通讯作者：
Vessella, RL
中科院分区：
文献类型：
--
作者：
Corey, E;Quinn, JE;Vessella, RL
背景。为了改善晚期前列腺癌（CaP）的治疗，建立模拟CaP骨转移的动物模型至关重要。与人类疾病不同，CaP异种移植模型很少自发地从原发肿瘤原位转移到骨。将LNCaP、PC-3、LuCaP 35、LuCaP 23.1 CaP细胞单细胞悬液直接注入SCID小鼠胫骨。免疫组织化学和骨组织形态计量学分析表征这些骨- cap模型。LuCaP 23.1产生成骨反应，LNCaP产生混合性病变，LuCaP 35和PC-3产生溶骨反应。我们在骨内生长的CaP细胞中检测到骨保护素、RANK配体、甲状旁腺激素相关蛋白和内皮素-1，这些蛋白与骨生长和重塑相关。这些动物模型可用于研究生物相互作用、途径和潜在的治疗靶点，也可用于评估治疗和预防CaP骨转移的新药物。(C) 2002 Wiley-Liss, Inc。
BACKGROUND. To improve the therapy of advanced prostate cancer (CaP), it is critical to develop animal models that mimic Cap bone metastases. Unlike the human disease, CaP xenograft models rarely metastasize spontaneously to bone from the orthotopic site of primary tumor growth.METHODS. Single-cell suspensions of LNCaP, PC-3, LuCaP 35, and LuCaP 23.1 CaP cells were injected directly into tibia of SCID mice. Immunohistochemistry and bone histomorphometrical analyses were performed to characterize these osseous-CaP models.RESULTS. LuCaP 23.1 Yields an osteoblastic response, LNCaP Yields mixed lesions, and LuCaP 35 and PC-3 result in osteolytic responses. We have detected osteoprotegerin, RANK ligand, parathyroid hormone-related protein, and endothelin-1, proteins associated with bone growth and remodeling, in the CaP cells grown in the bone.CONCLUSIONS. These animal models can be used to study biological interactions, pathways, and potential therapeutic targets, and also to evaluate new agents for treatment and prevention of CaP bone metastasis. (C) 2002 Wiley-Liss, Inc.