Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly), in Human Blood after Ingestion of Elastin Hydrolysate
Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly), in Human Blood after Ingestion of Elastin Hydrolysate
复制标题
DOI：
10.1021/jf300497p
复制
发表时间：
2012-05-23
影响因子：
6.1
通讯作者：
Sato, Kenji
中科院分区：
文献类型：
--
作者：
Shigemura, Yasutaka;Nakaba, Misako;Sato, Kenji
弹性蛋白水解物具有明显的有益效果，并且食物来源的肽脯氨酰甘氨酸（Pro-Gly）在口服摄入后存在于人体血液中。在健康人类志愿者摄入弹性蛋白水解产物（10 g/60 kg体重）后，使用外周血制备血浆样品，通过固相萃取从血浆样品中提取肽，并通过尺寸排阻色谱法（SEC）分级分离。SEC级分中的肽用异硫氰酸苯酯（PITC）衍生化并通过反相（RP）-HPLC解析。Pro-Gly是主要的食物来源的弹性蛋白肽，在摄入后30分钟达到最大值（18 μ M），并在摄入后4小时降至约20%。最后，在细胞培养中，培养基中Pro-Gly水平高于0.1 μ g/mL显著增强正常人皮肤成纤维细胞（NHDF）的弹性蛋白合成，而不影响细胞增殖速率。
Elastin hydrolysate has apparent beneficial effects, and the food-derived peptide prolyl-glycine (Pro-Gly) is present in human blood after oral ingestion. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reversed phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 mu M) at 30 min after ingestion, and decreasing to approximately 20% at 4 h after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 0.1 mu g/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.