Influence of nitric oxide on vascular resistance and muscle mechanics during tetanic contractions in situ
Influence of nitric oxide on vascular resistance and muscle mechanics during tetanic contractions in situ
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DOI：
10.1152/jappl.1999.87.1.142
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发表时间：
1999-07-01
影响因子：
3.3
通讯作者：
Provenzano, MA
中科院分区：
文献类型：
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作者：
Ameredes, BT;Provenzano, MA
在等长收缩血液灌注的犬腓肠肌-跖肌组中进行了一氧化氮（NO）合成抑制作用的研究。在重复性强直收缩期间（50 Hz 训练，200 ms 持续时间，1/s），使用 1 mM L-精氨酸琥珀酸 (L-ArgSA) 或 N-G-硝基-L-精氨酸甲酯 (L-NAME) 产生连续 NO 阻断期间，用泵控制肌肉血流量 (Q)。泵 Q 设置为与之前在每块肌肉的简短（3-5 分钟）控制收缩试验中测量的最大自发 Q（1.3-1.4 ml.min(-1).g(-1)）相匹配。在这些条件下，主动张力和摄氧量分别为500-600g/g和200-230μl.min(-1).g(-1)。 L-ArgSA 输注 3 分钟内，肌肉血管阻力 (R-v) 显着增加（从约 100 至 300 外周阻力单位；P < 0.05），而 L-NAME 时 R-v 增加幅度较小（从约 100 至 175 外周阻力单位；P < 0.05）。同时输注 0.5-10 mM L-精氨酸时，L-ArgSA 引起的 R-v 增加没有变化，但用 1-3 μg/ml 硝普钠则降低了 R-v (41-54%)。 L-NAME 引起的 R-v 增加被 1 mM L-精氨酸逆转。输注 L-ArgSA 会增加疲劳感；主动张力和肌内压降低了 62% 和 66%，而被动张力和基线肌内压分别增加了 80% 和 30%。这些数据表明，NO 在重复强直性收缩期间控制犬腓肠肌-跖肌内的 R-v 和收缩性方面可能发挥作用。
Studies of the effect of nitric oxide (NO) synthesis inhibition were performed in the isometrically contracting blood-perfused canine gastrocnemius-plantaris muscle group. Muscle blood flow (Q) was controlled with a pump during continuous NO blockade produced with either 1 mM L-argininosuccinic acid (L-ArgSA) or N-G-nitro-L-arginine methyl ester (L-NAME) during repetitive tetanic contractions (50-Hz trains, 200-ms duration, 1/s). Pump Q was set to match maximal spontaneous Q (1.3-1.4 ml.min(-1).g(-1)) measured in prior, brief (3-5 min) control contraction trials in each muscle. Active tension and oxygen uptake were 500-600 g/g and 200-230 mu l.min(-1).g(-1), respectively, under these conditions. Within 3 min of L-ArgSA infusion, vascular resistance across the muscle (R-v) increased significantly (from similar to 100 to 300 peripheral resistance units; P < 0.05), whereas R-v increased to a lesser extent with L-NAME (from similar to 100 to 175 peripheral resistance units; P < 0.05). The increase in R-v with L-ArgSA was unchanged by simultaneous infusion of 0.5-10 mM L-arginine but was reduced with 1-3 mu g/ml sodium nitroprusside (41-54%). The increase in R-v with L-NAME was reversed with 1 mM of L-arginine. Increased fatigue occurred with infusion of L-ArgSA; active tension and intramuscular pressure decreased by 62 and 66%, whereas passive tension and baseline intramuscular pressure increased by 80 and 30%, respectively. These data indicate a possible role for NO in the control of R-v and contractility within the canine gastrocnemius-plantaris muscle during repetitive tetanic contractions.