Telomerase and proliferative activity in placenta from women with and without fetal growth restriction
Telomerase and proliferative activity in placenta from women with and without fetal growth restriction
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DOI：
10.1016/s0029-7844(98)00383-4
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发表时间：
1999-01-01
影响因子：
7.2
通讯作者：
Nakagawara, K
中科院分区：
文献类型：
--
作者：
Izutsu, T;Kudo, T;Nakagawara, K
目的：方法：对30例早期妊娠绒毛组织（A组）和28例中晚期妊娠胎盘组织（B组）进行端粒酶活性检测。对11例不对称性FGR患者的胎盘组织进行端粒酶活性检测。结果：30例绒毛组织中28例（93.3%）端粒酶阳性，28例（64.3%）无FGR的胎盘组织中端粒酶阳性。相反，端粒重复序列扩增法检测11例不对称性FGR患者的胎盘组织中均未检测到端粒酶活性。原位端粒重复序列扩增法检测非FGR患者滋养层细胞端粒酶活性，而非对称性FGR患者滋养层细胞端粒酶活性未检测到。端粒酶活性在A、B组较C组明显增高（P <0.01）。A组和B组滋养层细胞的增殖活性率，明显表现为MIB-1染色阳性（分别为28.1 ± 1.7%和7.0 ± 2.9%）显著高于C组（1.9 +/- 0.6%; P < .01）。非对称性FGR患者胎盘中端粒酶和增殖活性极低，提示非对称性FGR患者胎盘衰老。(C)1999年由美国妇产科医师学会。
Objective: To analyze telomerase and proliferative activity in placenta from women with and without fetal growth restriction (FGR).Methods: Telomerase activity was analyzed in 30 first-trimester chorionic villi specimens (group A) and in 28 second- and third-trimester placenta specimens (group B) from women without FGR. Telomerase activity also was analyzed in 11 placenta specimens from women with asymmetric FGR (group C). The proliferative activity of these 69 specimens was assessed by immunohistochemical staining, using the MIB-1 monoclonal antibody.Results: Telomerase activity was detected in 28 (93.3%) of 30 chorionic villi specimens and in 18 (64.3%) of 28 placenta specimens without FGR. In contrast, no telomerase activity was exhibited in the placenta specimens from any of the 11 women with asymmetric FGR by telomeric repeat amplification protocol assay. Telomerase activity also was detected by in situ telomeric repeat amplification protocol assay in trophoblastic cells from women without FGR but not in trophoblastic cells from women with asymmetric FGR. Thus, telomerase activity was detected significantly more often in groups A and B than in group C (P < .01). The rate of proliferative activity, evident as positive MIB-1 staining in trophoblastic cells, in groups A and B (28.1 +/- 1.7% and 7.0 +/- 2.9%, respectively) was significantly higher than that in group C (1.9 +/- 0.6%; P < .01).Conclusion: Telomerase and proliferative activity were minimal in placenta from women with asymmetrical FGR, suggesting placental senescence with asymmetrical FGR.(C) 1999 by The American College of Obstetricians and Gynecologists.