Spermicidal activity of the safe natural antimicrobial peptide subtilosin.
Spermicidal activity of the safe natural antimicrobial peptide subtilosin.
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DOI：
10.1155/2008/540758
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Chikindas, Michael L
中科院分区：
文献类型：
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作者：
Sutyak, Katia E;Anderson, Robert A;Chikindas, Michael L
细菌性阴道病（BV）是一种每年影响数百万妇女的疾病，主要由革兰氏变菌阴道加德纳菌引起。据报道，与细菌性肠胃炎病例相关的一些生物体产生了多药耐药性，导致需要替代疗法。以前，我们报道了抗菌肽subtilosin已被证明对阴道乳杆菌有抗菌活性，但对被测试的健康阴道微生物群乳酸菌没有抗菌活性。在使用子宫颈外组织模型进行组织敏感性分析后，我们确定人类细胞在长时间暴露于部分纯化的subtilosin后仍能存活，这表明该化合物对人类使用是安全的。Subtilosin被证明以剂量依赖的方式消除人类精子的运动和向前发展，因此可以被认为是一种通用的杀精剂。这些结果表明，subtilosin将是一个有价值的成分，局部个人护理产品的目的是避孕和BV的预防和治疗。
Bacterial vaginosis (BV), a condition affecting millions of women each year, is primarily caused by the gram-variable organism Gardnerella vaginalis. A number of organisms associated with BV cases have been reported to develop multidrug resistance, leading to the need for alternative therapies. Previously, we reported the antimicrobial peptide subtilosin has proven antimicrobial activity against G. vaginalis, but not against the tested healthy vaginal microbiota of lactobacilli. After conducting tissue sensitivity assays using an ectocervical tissue model, we determined that human cells remained viable after prolonged exposures to partially-purified subtilosin, indicating the compound is safe for human use. Subtilosin was shown to eliminate the motility and forward progression of human spermatozoa in a dose-dependent manner, and can therefore be considered a general spermicidal agent. These results suggest subtilosin would be a valuable component in topical personal care products aimed at contraception and BV prophylaxis and treatment.