Polyethylene Glycol-Based Synthetic Hydrogel Sealant for Closing Vitrectomy Wounds: An In Vivo and Histological Study.
Polyethylene Glycol-Based Synthetic Hydrogel Sealant for Closing Vitrectomy Wounds: An In Vivo and Histological Study.
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DOI：
10.1167/tvst.5.3.7
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发表时间：
2016-05
影响因子：
3
通讯作者：
Oshika T
中科院分区：
文献类型：
--
作者：
Hoshi S;Okamoto F;Arai M;Hirose T;Fukuda S;Sugiura Y;Oshika T
我们使用荷兰有色兔眼进行了一项体内研究，以测试聚乙二醇（PEG）密封剂在微切口玻璃体切除术（MIVS）中用于闭合无缝线巩膜切口的有效性。在兔眼上进行三孔，23G玻璃体切除术。通过在巩膜切开术部位应用0.625%聚维酮碘确认漏气后，使用27号针通过结膜切口结膜下注射PEG密封剂以覆盖巩膜切开术伤口，然后通过应用氙气灯60秒使其聚合。在手术前一天和手术后1、3、5和7天进行眼科检查和眼内压测量。手术后7天摘除眼睛进行组织学评价。在结膜下注射后，通过应用氙气灯使PEG密封剂快速聚合，并且在所有情况下，其牢固地密封巩膜切口而没有空气泄漏，如通过聚维酮碘滴注所证实的。使用PEG密封剂闭合的结膜和巩膜伤口成功附着，并保持完整，直至随访期结束。所有患眼均无术后低眼压或感染的迹象，未发现PEG密封剂的不良反应。在组织学检查中，在巩膜切开部位观察到线性瘢痕形成和胶原纤维嗜酸性染色，而巩膜切开隧道似乎紧密闭合。PEG密封剂可用于闭合兔的免缝合23号玻璃体切除术切口。PEG密封剂可能成为闭合玻璃体切除术切口（包括儿科病例）的有效选择。
We conducted an in vivo study using Dutch pigmented rabbit eyes to test the usefulness of polyethylene glycol (PEG) sealant for the closure of sutureless sclerotomies in microincisional vitrectomy surgery (MIVS). Three-port, 23-gauge vitrectomy was performed on rabbit eyes. After air leakage was confirmed by the application of 0.625% povidone–iodine at the sclerotomy site, PEG sealant was subconjunctivally injected using a 27-gauge needle through conjunctival incisions to cover the sclerotomy wounds, following which it was polymerized by the application of xenon light for 60 seconds. Ophthalmological examinations and intraocular pressure measurements were conducted the day before and 1, 3, 5, and 7 days after surgery. The eyes were enucleated for histological evaluation 7 days after surgery. PEG sealant was rapidly polymerized by the application of xenon light after subconjunctival injection, and it firmly sealed the sclerotomies without air leakage, as confirmed by povidone–iodine dropping, in all cases. Conjunctival and scleral wounds closed with PEG sealant were successfully attached and remained intact till the end of the follow-up period. There was no sign of postoperative hypotony or infection in any eye, and no adverse effects of PEG sealant were found. In histological examination, linear scar formation and eosinophilic staining of collagen fibers were observed at the sclerotomy sites, while the sclerotomy tunnels appeared tightly closed. PEG sealant can be useful for the closure of sutureless 23-gauge vitrectomy incisions in rabbits. The PEG sealant may become an effective option for closing vitrectomy incisions including pediatric cases.