Transcriptome Profiling of Duodenum Reveals the Importance of Boron Supplementation in Modulating Immune Activities in Rats
Transcriptome Profiling of Duodenum Reveals the Importance of Boron Supplementation in Modulating Immune Activities in Rats
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DOI：
10.1007/s12011-021-02983-w
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发表时间：
2021-11
影响因子：
3.9
通讯作者：
Chunfang Zhao;Yujiao Han;Chenfang Wang;M. Ren;Qianqian Hu;Youfang Gu;Pengfei Ye;Shenghe Li;Erhui Jin
中科院分区：
文献类型：
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作者：
Chunfang Zhao;Yujiao Han;Chenfang Wang;M. Ren;Qianqian Hu;Youfang Gu;Pengfei Ye;Shenghe Li;Erhui Jin
硼是动物必需的微量元素，适量补充硼可提高动物的免疫功能。为了说明硼在大鼠模型中的作用，在用不同浓度的硼处理后对来自十二指肠的RNA进行RNA-Seq，其中硼以硼酸的形式给予。在0、10和320 mg/L硼（0、57.21和1830.66 mg/L硼酸）处理组中获得了超过4700万个读数，分别产生了58 965 402、48 607 328和46 760 660个干净读数。超过95%的干净读段与大鼠参考基因组成功匹配，并组装生成32662个转录本。在0与10和0与320 mg/L硼比较组之间，共发现624和391个差异表达的候选基因（DEG）。我们还确定了转录起始位点、转录终止位点和跳过外显子是主要的选择性剪接事件。GO注释显示大部分DEG参与免疫活性的调节。DEG在甲型流感病毒、单纯疱疹病毒感染、胞质DNA传感途径以及抗原加工和呈递信号传导途径中富集。在这些信号通路中富集的基因的表达水平表明，较低剂量的硼可以实现更好的促进十二指肠免疫反应的效果。这些对免疫系统的影响似乎是通过改变参与相关信号传导途径的基因的表达模式以剂量依赖性模式介导的。这些数据提供了更多的见解免疫调节的分子机制，大鼠在饮食硼治疗。
As an essential trace element, appropriate boron supplementation can promote immune function of animals. To illustrate the effects of boron in a rat model, RNA-Seq was conducted for the RNA from duodenum after treatment with different concentration of boron in which boron was given in the form of boric acid. More than 47 million reads were obtained in 0, 10, and 320 mg/L boron (0, 57.21, and 1830.66 mg/L boric acid) treatment groups that produced 58 965 402, 48 607 328, and 46 760 660 clean reads, respectively. More than 95% of the clean reads were successfully matched to the rat reference genome and assembled to generate 32 662 transcripts. A total of 624 and 391 differentially expressed candidate genes (DEGs) were found between 0 vs.10 and 0 vs. 320 mg/L boron comparison groups. We also identified transcription start site, transcription terminal site, and skipped exons as the main alternative splicing events. GO annotations revealed most of DEGs were involved in the regulation of immune activity. The DEGs were enriched in influenza A, herpes simplex infection, cytosolic DNA-sensing pathway, and antigen processing and presentation signaling pathways. The expression levels of genes enriched in these signaling pathways indicate that lower doses of boron could achieve better effects on promoting immune response in the duodenum. These effects on the immune system appear to be mediated via altering the expression patterns of genes involved in the related signaling pathways in a dose-dependent pattern. These data provide more insights into the molecular mechanisms of immune regulation in rats in response to dietary boron treatment.