Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand
Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand
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DOI：
10.1016/s0035-9203(99)90120-7
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发表时间：
1999-07-01
影响因子：
2.2
通讯作者：
Viriyakosol, S
中科院分区：
文献类型：
--
作者：
Snounou, G;Zhu, XP;Viriyakosol, S
恶性疟原虫分离株获得从泰国患者参加一个疟疾诊所在泰国-柬埔寨边境超过4个横截面调查进行了S-每月的间隔。采用巢式聚合酶链反应（PCR）扩增恶性疟原虫3个抗原基因（msp 1、msp 2和glurp）的多态性区域，确定恶性疟原虫种群的遗传结构。虽然高度的多样性特点，这些分离株，与专利疟疾感染的寄生虫的整体人口结构观察到保持相对稳定的时间。最高程度的多态性与msp 2，和线的平均数，每次感染（感染复数）计算与此标志物是高于使用msp 1或glurp单独获得，或组合。在76%的样本中发现了大于或等于2条寄生虫线的感染，并且在雨季开始和结束时比例更高。观察到随机分布的两个有趣的例外，涉及2个等位基因变体，在一个病例中发现分离的（msp 1 MAD 20-家族），另一个病例中发现相关的（msp 2 FC 27-家族）。这些类型的数据的流行病学意义进行了讨论。
Plasmodium falciparum isolates were obtained from Thai patients attending a malaria clinic on the Thai-Kampuchean border over 4 cross-sectional surveys carried out at S-monthly intervals. The genetic structure of the parasite populations was determined by nested polymerase chain reaction (PCR) amplification of polymorphic regions of 3 P. falciparum antigen genes: msp1, msp2 and glurp. Although a high degree of diversity characterized these isolates, the overall population structure of the parasites associated with patent malaria infections was observed to remain relatively stable over time. The highest degree of polymorphism was observed with msp2, and the mean number of lines per infection (multiplicity of infection) calculated with this marker was higher than that obtained using msp1 or glurp alone, or combined. Infections with greater than or equal to 2 parasite lines were seen in 76% of the samples, and were proportionally more numerous at the start and end of the rainy season. Two interesting exceptions to the random distribution were observed and involved 2 allelic variants which in one case were found dissociated (msp1 MAD20-family) and in the other were associated (msp2 FC27-family). The epidemiological significance of these types of data is discussed.