Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry.
Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry.
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DOI：
10.1016/j.aca.2007.12.019
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发表时间：
2008-02
影响因子：
6.2
通讯作者：
C. S. Thompson;S. Haughey;I. Traynor;T. Fodey;C. Elliott;J. Antignac;B. Le Bizec;S. Crooks
中科院分区：
文献类型：
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作者：
C. S. Thompson;S. Haughey;I. Traynor;T. Fodey;C. Elliott;J. Antignac;B. Le Bizec;S. Crooks
莱克多巴胺是β-2激动剂(β-激动剂)家族的一员。它在包括美国和加拿大在内的全球20多个国家获得了作为动物生长促进剂的许可，但没有获得许可或被其他150多个国家禁止，包括欧盟内部的国家。在从美国运来的猪肉中发现RCT的痕迹后，中华人民共和国中国决定禁止从一些加工厂进口猪肉，在供人食用的牲畜中使用RCT的问题最近变得突出起来。为了监测欧洲境内非法使用此类化合物的情况，需要有一个强大而可靠的检测方案，能够检测出低浓度的RCT。本研究建立了一种光学生物传感器的筛选方法。将所建立的方法与LC-MS/MS确证方法进行了比较。这些方法被用来研究治疗后检测猪RCT的能力。两种检测方法均能检测到尿液和肝脏中低浓度的μ、GKG、−。肝脏被发现是不太适合的样本基质，停药5天后无法检测到RCT残留量。然而，尿样在停药几周后仍含有可检测到的RCT残留物。该传感器与LC-MS/MS方法对尿样和肝脏样品的相关系数(R2)分别为0.99和0.97。结论是，基于肝脏RCT分析的检测制度比基于尿液分析的检测制度更不可能发现非法给药。尿样为检测戒断后较长时间内的RCT残留提供了极好的基质。
Ractopamine (RCT) is a member of the β-2-agonist (β-agonist) family. It is licensed for use as an animal growth promoter in more than 20 countries worldwide, including the United States and Canada, but is either not licensed or prohibited by over 150 others, including those within the European Union. The issue of the use of RCT in livestock bound for human consumption has risen to prominence recently following the decision by The People's Republic of China to ban the import of pork from a number of processing plants after finding traces of RCT in shipments from the U.S.A. In order to monitor for the illegal use of such compounds within Europe, there is a requirement to have a robust and reliable testing scheme capable of the detection of low concentrations of RCT. In the present study an optical biosensor screening assay was developed. The developed assay was compared with a liquid chromatography/mass spectrometry/mass spectrometry (LC–MS/MS) confirmatory procedure. These methods were used to study the ability to detect RCT in pigs following treatment. Both testing procedures were capable of detecting low μgkg−1concentrations of the drug in urine and liver. Liver was found to be a less suitable sample matrix, with RCT residue levels being undetectable after 5 days withdrawal of the drug. Urine samples however still contained detectable RCT residues several weeks after withdrawal. The correlation (as measured by r2) between the biosensor and LC–MS/MS methods was 0.99 and 0.97 for urine and liver samples, respectively. It is concluded that testing regimes based on RCT analysis in liver are less likely to detect illegal administration of the drug than those based on urine analysis. Urine samples provide an excellent matrix for the detection of RCT residues for an extended period post withdrawal.