Antifungal effect of ophthalmic preservatives phenylmercuric nitrate and benzalkonium chloride on ocular pathogenic filamentous fungi
Antifungal effect of ophthalmic preservatives phenylmercuric nitrate and benzalkonium chloride on ocular pathogenic filamentous fungi
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眼用防腐剂硝酸苯汞和苯扎氯铵对眼部致病丝状真菌的抗真菌作用
DOI：
10.1016/j.diagmicrobio.2012.09.008
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Pang, Guangren
中科院分区：
文献类型：
--
作者：
Xu, Yan;He, Yi;Pang, Guangren
在本研究中，硝酸苯汞和苯扎氯铵与那他霉素和酮康唑的抗真菌作用进行了评估，对216丝状真菌分离的真菌性角膜炎的情况下。它们包括112个镰刀菌分离物，94个曲霉分离物，和10个链格孢分离物。通过临床和实验室标准研究所M38-A文件批准的丝状真菌的肉汤稀释抗真菌药敏试验对菌株进行测试。结果表明，硝酸苯汞对镰刀菌的MIC_（50）分别为0.0156、0.0156和0.0313 μ g/mL，曲霉属，和A. alternata，分别。硝酸苯汞对镰刀菌属的MIC 90值分别为0.0313、0.0313和0.0313 μ g/mL，曲霉属，和A. alternata，分别。苯扎氯铵对镰刀菌属的MIC 50值分别为16、32和8 μ g/mL，曲霉属，和A. alternata，分别。苯扎氯铵对镰刀菌属的MIC 90值分别为32、32和16 μ g/mL，曲霉属，和A. alternata，分别。结果表明，硝酸苯汞具有较强的抗真菌活性，其体外抗眼部丝状真菌的效果明显上级苯扎氯铵、那他霉素和酮康唑，可作为治疗真菌性角膜炎的主要药物进行进一步研究。(C)2013 Elsevier Inc. All rights reserved.
In the present study, the antifungal effects of phenylmercuric nitrate and benzalkonium chloride versus those of natamycin and ketoconazole were assessed against 216 filamentous fungi isolates from cases of fungal keratitis. They included 112 Fusarium isolates, 94 Aspergillus isolates, and 10 Alternaria alternata isolates. The strains were tested by broth dilution antifungal susceptibility testing of filamentous fungi approved by the Clinical and Laboratory Standards Institute M38-A document. The results showed that the MIC50 values of phenylmercuric nitrate were 0.0156, 0.0156, and 0.0313 mu g/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC90 values of phenylmercuric nitrate were 0.0313, 0.0313, and 0.0313 mu g/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC50 values of benzalkonium chloride were 16, 32, and 8 mu g/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC90 values of benzalkonium chloride were 32, 32, and 16 mu g/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The study indicates that phenylmercuric nitrate has considerable antifungal activity and its effect is significantly superior to those of benzalkonium chloride, natamycin, and ketoconazole against ocular pathogenic filamentous fungi in vitro, deserving further investigation for treating fungal keratitis as a main drug. (C) 2013 Elsevier Inc. All rights reserved.