Common Assays in Mammalian Golgi Studies.
Common Assays in Mammalian Golgi Studies.
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DOI：
10.1007/978-1-0716-2639-9_20
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发表时间：
2023
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高尔基体是一个复杂的结构，其特征是层叠排列紧密的扁平脑池。在哺乳动物细胞中，高尔基体堆积通常集中在核周区域，并连接在一起形成带状。这种结构是动态的，以适应双向进出高尔基体的连续货物流动，并在生理和病理条件下经历形态变化。这种精细的、堆叠的高尔基结构使其很难用传统的光学显微镜甚至超分辨率显微镜进行研究。此外，由于维持复杂和动态高尔基体结构的蛋白质机制的知识空白，理解高尔基体结构动力学如何影响细胞过程的努力一直很缓慢。在这篇方法文章中，我们列出了我们研究中使用的常见分析方法，以帮助新的和现有的研究人员选择最合适的方法来适当地解决他们的问题。
The Golgi is a complex structure characterized by stacks of tightly aligned flat cisternae. In mammalian cells, Golgi stacks often concentrate in the perinuclear region and link together to form a ribbon. This structure is dynamic to accommodate continuous cargo flow in and out of the Golgi in both directions and undergoes morphological changes under physiological and pathological conditions. The fine, stacked Golgi structure makes it difficult to study by conventional light or even super-resolution microscopy. Furthermore, efforts to understand how Golgi structural dynamics impact cellular processes have been slow because of the knowledge gap in the protein machinery that maintains the complex and dynamic Golgi structure. In this method article, we list the common assays used in our research to help new and established researchers select the most appropriate method to properly address their questions.