Cafeteria diet increases fat mass and chronically elevates lumbar sympathetic nerve activity in rats.
Cafeteria diet increases fat mass and chronically elevates lumbar sympathetic nerve activity in rats.
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DOI：
10.1161/hypertensionaha.112.194886
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Ajasin D
中科院分区：
文献类型：
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作者：
Muntzel MS;Al-Naimi OA;Barclay A;Ajasin D
肥胖引起交感神经激活，促进动脉粥样硬化、终末器官损伤和高血压。由于高脂肪诱导的大鼠体重增加在高热量饮食开始后1-3天升高血浆瘦素，我们假设饮食诱导的过度喂养将在该方案开始后一周内增加交感神经活性。为了验证这一点，我们连续测量交感神经活动和血压之前和期间的饮食诱导的肥胖使用高热量的饮食风格的饮食。雌性Wistar大鼠，其中无线电遥测仪已被植入连续监测腰交感神经活动，平均动脉压和心率，随机分配到组，接受定期的食物（对照）或食堂饮食的一段时间为15天。这种短期的动物饲料喂养方案导致体重适度但不显著的增加（P = 0.07），棕色和白色脂肪组织加倍（P < 0.01）。脂肪量的增加伴随着血浆瘦素的升高（P < 0.001），但葡萄糖没有变化。与对照组相比，自助餐组大鼠的总体心率和血压均较高（P < 0.05）。食堂饮食诱导的体重增加导致腰交感神经活动增加，在饮食的第12天变得显著（p < 0.001）。这些数据首次表明，高脂饮食刺激大鼠腰交感神经驱动持续增加。
Obesity causes sympathetic activation that promotes atherosclerosis, end-organ damage, and hypertension. Because high-fat induced weight gain in rats elevates plasma leptin at 1–3 days following onset of calorie dense diets, we hypothesized that diet-induced overfeeding will increase sympathetic activity within one week following onset of the regimen. To test this, we continuously measured sympathetic activity and blood pressure before and during the onset of diet-induced obesity using a high calorie cafeteria-style diet. Female Wistar rats, in which radiotelemeters had been implanted for continuous monitoring of lumbar sympathetic activity, mean arterial pressure, and heart rate, were randomly assigned to groups that received regular chow (control) or a cafeteria diet for a period of 15 days. This short-term cafeteria-feeding regimen caused modest but non-significant increases in body weight (P = 0.07) and a doubling of brown and white adipose tissue (P < 0.01). The increases in fat mass were accompanied by elevations in plasma leptin (P < 0.001) but no change in glucose. Overall heart rates and blood pressure were higher in cafeteria rats compared with controls (P < 0.05). Cafeteria diet-induced weight gain caused increases in lumbar sympathetic nerve activity that became significant by the 12th day of the diet (p < 0.001). These data show, for the first time, that the high-fat cafeteria-style diet stimulates sustained increases in lumbar sympathetic neural drive in rats.