Gene expression profiling of Epstein-Barr virus-positive and -negative monomorphic B-cell posttransplant lymphoproliferative disorders
Gene expression profiling of Epstein-Barr virus-positive and -negative monomorphic B-cell posttransplant lymphoproliferative disorders
复制标题
DOI：
10.1097/pdm.0b013e31804f54a9
复制
发表时间：
2007-09-01
影响因子：
--
通讯作者：
Swerdlow, Steven H.
中科院分区：
文献类型：
--
作者：
Craig, Fiona E.;Johnson, Lawrence R.;Swerdlow, Steven H.
虽然大多数移植后淋巴组织增生性疾病（PTLD）与EB病毒（EBV）感染有关，但约20%缺乏可检测的EBV（EBV-）。目前尚不确定后者是否真正不同于EBV+ PTLD或可能与另一种感染因子有关。本研究利用基因表达谱分析进一步研究EBV+和EBV-单形性B细胞PTLD之间的关系，并寻找其发病机制的线索。使用Affyestive HU 133 A基因芯片比较4例EBV+和4例EBV -单形B细胞PTLD病例。分层聚类成功区分了EB病毒+和EB病毒-组。相对于EBV-PTLD，54个转录本在EBV + PTLD中过表达。鉴定的转录物包括IRF 7（EBV LMP 1表达的已知调节物）、EBI 2（EBV诱导的基因2）和干扰素诱导的3（MX 1、IFITM 1和IFITM 3）。此外，EBV+组含有232个相对于EBV-组减少的转录物，包括与先前报道的在EBV感染培养的B细胞系后的那些一致的变化。总之，在一小组单形性B细胞PTLD中，EBV +病例显示了与B细胞的EBV感染相关的基因表达变化的子集。相比之下，EBV-PTLD缺乏病毒相关的变化，表明它们在生物学上是不同的。
Although most posttransplant lymphoproliferative disorders (PTLD) are related to Epstein-Barr virus (EBV) infection, approximately 20% lack detectable EBV (EBV-). It is uncertain whether the latter cases are truly distinct from EBV+ PTLD or possibly relate to another infectious agent. This study used gene expression profiling to further investigate the relationship between EBV+ and EBV- monomorphic B-cell PTLD, and to search for clues to their pathogenesis. Affymetrix HU133A GeneChips were used to compare 4 EBV+ and 4 EBV - cases of monomorphic B-cell PTLD. Hierarchical clustering successfully distinguished the EBV+ and EBV - groups. Relative to EBV - PTLD, 54 transcripts were over-expressed in EBV + PTLD. The transcripts identified included IRF7 (a known regulator of EBV LMP1 expression), EBI2 (EBV-induced gene 2), and 3 that are interferon induced (MX1, IFITM1, and IFITM3). In addition, the EBV+ group contained 232 transcripts decreased relative to the EBV- group, including changes concordant with those previously reported after EBV infection of cultured B-cell lines. In summary, in a small group of monomorphic B-cell PTLD, EBV + cases demonstrated a subset of gene expression changes associated with EBV infection of B cells. By contrast, EBV - PTLD lacked viral-associated changes suggesting that they are biologically distinct.