Three-micrometer-diameter needle electrode with an amplifier for extracellular in vivo recordings
Three-micrometer-diameter needle electrode with an amplifier for extracellular in vivo recordings
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带有放大器的三微米直径针电极，用于细胞外体内记录
DOI：
10.1073/pnas.2008233118
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kawano Takeshi
中科院分区：
文献类型：
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作者：
Kita Yuto;Tsuruhara Shuhei;Kubo Hiroshi;Yamashita Koji;Seikoba Yu;Idogawa Shinnosuke;Sawahata Hirohito;Yamagiwa Shota;Leong Xian Long Angela;Numano Rika;Koida Kowa;Kawano Takeshi
微型针电极装置在脑组织中提供神经元信号记录能力；然而，为了最大限度地减少组织损伤，使用较小几何形状的针会导致电性能下降，包括高电阻抗和低信噪比（SNR）记录。我们使用一种器件组装技术克服了这些限制，该技术在1 × 1 mm2的器件内使用一个称为STACK的单针顶放大器封装。基于硅（Si）生长技术，在硅块上制备了尖端直径<3 μ m、长度为400 μ m的针电极作为模块。通过将针电极叠加在放大器的另一个模块上，改善了针电极的高阻抗特性。STACK装置的电压增益为>.98 (- 0.175 dB)，能够记录小鼠大脑的局部场电位和动作电位，信噪比提高到6.2。此外，该设备允许我们使用蓝牙模块来演示这些神经元信号的无线记录；慢性实验也采用stack植入小鼠进行。
Microscale needle-electrode devices offer neuronal signal recording capability in brain tissue; however, using needles of smaller geometry to minimize tissue damage causes degradation of electrical properties, including high electrical impedance and low signal-to-noise ratio (SNR) recording. We overcome these limitations using a device assembly technique that uses a single needle-topped amplifier package, called STACK, within a device of ∼1 × 1 mm2. Based on silicon (Si) growth technology, a <3-µm-tip-diameter, 400-µm-length needle electrode was fabricated on a Si block as the module. The high electrical impedance characteristics of the needle electrode were improved by stacking it on the other module of the amplifier. The STACK device exhibited a voltage gain of >0.98 (−0.175 dB), enabling recording of the local field potential and action potentials from the mouse brain in vivo with an improved SNR of 6.2. Additionally, the device allowed us to use a Bluetooth module to demonstrate wireless recording of these neuronal signals; the chronic experiment was also conducted using STACK-implanted mice.