Encapsulation of bacteriophages in whey protein films for extended storage and release
Encapsulation of bacteriophages in whey protein films for extended storage and release
复制标题
将噬菌体封装在乳清蛋白膜中以延长储存和释放
DOI：
10.1016/j.foodhyd.2013.09.017
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发表时间：
2014
影响因子：
10.7
通讯作者：
N. Nitin
中科院分区：
文献类型：
--
作者：
Erica L Vonasek;Phuong Le;N. Nitin
本研究的总体动机是使病原体特异性抗菌包装材料的发展。这些材料有望在提高食品材料的抗菌处理效果和共生菌和益生菌的保留方面产生重大影响。为了实现这些目标，本研究旨在将模型噬菌体T4噬菌体封装在基于WPI的可食用蛋白薄膜中。研究了噬菌体包被WPI膜的稳定性和包被噬菌体的释放特性。采用微生物生长抑制试验对噬菌体包封可食性薄膜的抑菌效果进行了研究。用共聚焦荧光显微镜测定了噬菌体在食用膜中的分布。结果表明，WPI膜能够在环境（22°c和光照）和冷藏（4°c和黑暗）条件下稳定噬菌体，而在一个月的时间内噬菌体的感染性没有明显损失。此外，WPI膜能够在3小时内在水环境和叶片表面释放显著浓度的噬菌体。抗菌活性测定表明，包裹WPI膜的噬菌体能有效抑制微生物的生长。微生物生长分析结果显示，对照样品和处理样品之间的微生物水平相差约5 log。共聚焦成像测量显示，荧光标记的噬菌体均匀分布在WPI膜基质内。总之，本研究展示了噬菌体与可食用包装材料的整合，以开发新的生物防治活性包装材料。
The overall motivation of this study was to enable development of pathogen specific antimicrobial packaging materials. These materials are expected to have a significant impact in improving efficacy of antimicrobial treatment and retention of commensal and probiotic microbes on food materials. To achieve these goals, this study was aimed at encapsulation of a model bacteriophage, T4 bacteriophage, in WPI based edible protein films. Phage encapsulated WPI films were characterized for stability and release of encapsulated phages. Antimicrobial efficacy of phage encapsulating edible films was characterized using a microbial growth inhibition assay. Distribution of phages in edible film was measured using a confocal fluorescence microscopy. The results demonstrate that the WPI films are able to stabilize phages at ambient (22 °c and light) and refrigerated (4 °C and dark) conditions without significant loss in phage infectivity over a period of one month. Additionally, the WPI films are able to release a significant concentration of phages in an aqueous environment and leaf surface within 3 h of incubation. Antimicrobial activity measurements demonstrate that the phage encapsulating WPI film can effectively inhibit the microbial growth. The results of microbial growth analysis showed an approximately 5 log difference in microbial levels between the control and the treatment samples. Confocal imaging measurements show that fluorescently labeled phages are homogenously distributed within the WPI film matrix. Overall, this study demonstrates integration of phages with edible packaging materials to develop novel active packaging materials for biocontrol applications.