Cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group
Cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group
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DOI：
10.4269/ajtmh.2002.66.804
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发表时间：
2002-06-01
影响因子：
3.3
通讯作者：
Coetzee, M
中科院分区：
文献类型：
--
作者：
Koekemoer, LL;Kamau, L;Coetzee, M
不死按蚊（Anopheles funestus Giles）是非洲主要的疟疾媒介，属于一组形态特征相似的种。A.成员的形态学鉴定funestus组是困难的，因为在成虫或未成熟阶段的区别特征重叠，以及必须培育同雌系来检查幼虫和卵的特征。建立了一种快速的rDNA聚合酶链反应（PCR）方法，用于准确鉴定A. funestus集团该PCR是基于rDNA上ITS2区域的种特异性引物来鉴定A.结果表明，该基因的核苷酸序列为505bp，vaneedeni吉利斯and Coetzee（587bp），rivulorum Leeson（411bp），leesoni Evans（146bp），parensis吉利斯（252bp）。
Anopheles funestus Giles is a major malaria vector in Africa belonging to a group of species with mor-phologically similar characteristics. Morphological identification of members of the A. funestus group is difficult because of overlap of distinguishing characteristics in adult or immature stages as well as the necessity to rear isofemale lines to examine larval and egg characters. A rapid rDNA polymerase chain reaction (PCR) method has been developed to accurately identify five members of the A. funestus group. This PCR is based on species-specific primers in the ITS2 region on the rDNA to identify A. funestus (approximate to505bp), Anopheles vaneedeni Gillies and Coetzee (approximate to587bp), Anopheles rivulorum Leeson (approximate to411bp), Anopheles leesoni Evans (approximate to146bp), and Anopheles parensis Gillies (approximate to252bp).