Growth of Protein Crystal at Interface between Two Liquids Using Slow Cooling Method
Growth of Protein Crystal at Interface between Two Liquids Using Slow Cooling Method
复制标题
慢冷法在两种液体界面生长蛋白质晶体
DOI：
10.1143/jjap.41.l726
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发表时间：
2002
影响因子：
1.5
通讯作者：
T. Sasaki
中科院分区：
文献类型：
--
作者：
H. Adachi;Takahiro Watanabe;M. Yoshimura;Y. Mori;T. Sasaki
一种新的方法已经发展到生长高质量和大的蛋白质晶体。通过缓慢冷却方法，在两种液体的界面处从自发成核生长四氢溶菌酶晶体，其中晶体不与生长容器接触。晶体的狭长形状表明它们是在较低的过饱和度下生长的，从而导致高结晶度。这种方法还可以很容易地去除晶体，而不会造成任何机械损坏。在25天内从保持在界面处的晶种获得了长度为2.5mm的大溶菌酶晶体。在两种液体之间的界面处生长和受控缓慢冷却的组合对于高质量和大的蛋白质晶体的生长是有效的。
A new method has been developed to grow high-quality and large protein crystals. By a slow cooling method, tetragonal lysozyme crystals were grown from spontaneous nucleation at the interface of two liquids wherein the crystals had no contact with a growth vessel. The long and narrow shape of the crystals indicated that they had grown at a lower supersaturation, leading to high crystallinity. This method also made it possible to remove the crystals easily without causing any mechanical damage. A large lysozyme crystal 2.5 mm in length was obtained in 25 days from a seed crystal kept at the interface. The combination of growth at the interface between two liquids and controlled slow cooling is effective for the growth of high-quality and large protein crystals.