Isolation of arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes
Isolation of arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes
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DOI：
10.1105/tpc.8.2.241
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发表时间：
1996-02-01
期刊：
影响因子：
11.6
通讯作者：
Ausubel, FM
中科院分区：
文献类型：
--
作者：
Reuber, TL;Ausubel, FM
拟南芥抗病基因RPS2参与识别携带无毒基因avrRpt2的细菌病原菌，RPM1基因参与识别携带avrRpm1或avrB的病原菌。我们鉴定并克隆了两个拟南芥基因AIG1和AIG2(avrRpt2诱导基因)，它们在感染携带avrRpt2的拟南芥斑点假单胞菌ES4326菌株后早期表现出RPS2和avrRpt2依赖的诱导。但携带avrRpm1或avrB基因的ES4326不能诱导AIG1和AIG2的早期表达。相反，携带avrRpm1或avrB的ES4326诱导了先前分离的防御相关基因ELI3的早期表达，而携带avrRpt2的ES4326则没有。AIG基因和ELI3的诱导模式表明，不同的抗性基因-AVR基因组合可以诱导不同的防御反应。此外，通过检测携带avrRpt2的ES4326和携带avrRpm1的ES4326混合接种的植株中AIG1和ELI3的表达，我们发现RPS2和RPM1介导的抗性反应之间存在干扰。
The Arabidopsis disease resistance gene RPS2 is involved in recognition of bacterial pathogens carrying the avirulence gene avrRpt2, and the RPM1 resistance gene is involved in recognition of pathogens carrying avrRpm1 or avrB. We identified and cloned two Arabidopsis genes, AIG1 and AIG2 (for avrRpt2-induced gene), that exhibit RPS2- and avrRpt2-dependent induction early after infection with Pseodomonas syringae pv maculicola strain ES4326 carrying avrRpt2. However, ES4326 carrying avrRpm1 or avrB did not induce early expression of AIG1 and AIG2. Conversely, ES4326 carrying avrRpm1 or avrB induced early expression of the previously isolated defense-related gene ELI3, whereas ES4326 carrying avrRpt2 did not, The induction patterns of the AIG genes and ELI3 demonstrate that different resistance gene-avr gene combinations can elicit distinct defense responses. Furthermore, by examining the expression of AIG1 and ELI3 in plants infiltrated with a mixed inoculum of ES4326 carrying avrRpt2 and ES4326 carrying avrRpm1, we found that there is interference between the RPS2- and RPM1-mediated resistance responses.