Different roles suggested by sex-biased expression and pheromone binding affinity among three pheromone binding proteins in the pink rice borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae)
Different roles suggested by sex-biased expression and pheromone binding affinity among three pheromone binding proteins in the pink rice borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae)
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粉红螟、大螟（鳞翅目：夜蛾科）三种信息素结合蛋白的性别偏向表达和信息素结合亲和力表明不同的作用
DOI：
10.1016/j.jinsphys.2014.05.013
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发表时间：
2014-07-01
影响因子：
2.2
通讯作者：
Dong, Shuang-Lin
中科院分区：
文献类型：
--
作者：
Jin, Jun-Yan;Li, Zhao-Qun;Dong, Shuang-Lin
信息素结合蛋白（Pheromone binding proteins，PBPs）被认为是通过感觉淋巴液将疏水性信息素分子结合并转运到嗅觉神经元树突膜上的特异性信息素受体上的蛋白。目前已鉴定出3个PBP基因，每个基因与该科其他物种的PBP基因具有高度的同源性。3种蛋白质的功能差异知之甚少。本研究首次从水稻二化螟Sesamia inferens中克隆到3个PBP cDNA（SinfPBP 1、2、3）。实时荧光定量PCR结果显示，3种PBP在时间上相似，但在性别上有很大差异。SinfPBP 1和SinfPBP 2的表达分别是高度和中度雄性偏向的，而SinfPBP 3是轻微雌性偏向的，因为SinfPBPs在雄性触角中以非常不同的水平（PBP 1> PBP 2>> PBP 3）表达，但在雌性触角中以相似的水平表达。此外，在荧光竞争结合试验中，3种SinfPBPs显示不同的配体结合谱。SinfPBP_1对所有3种性信息素组分都表现出很高且相似的结合亲和力（Ki = 0.72-1.60 μ M），而SinfPBP_2对醇和醛组分表现出选择性结合（Ki = 0.78-1.71 μ M），而SinfPBP_3对3种性信息素组分表现出不明显的结合。这些结果表明，SinfPBP 1在中华绒螯蟹雌虫性信息素的接收中起主要作用。推断，而SinfPBP 3起最小的作用（如果有的话），而SinfPBP 2起识别醇和醛组分的作用。(C)2014爱思唯尔有限公司版权所有。
Pheromone binding proteins (PBPs) are thought to bind and transport hydrophobic sex pheromone molecules across the aqueous sensillar lymph to specific pheromone receptors on the dendritic membrane of olfactory neurons. A maximum of 3 PBP genes have been consistently identified in noctuid species, and each of them shares high identity with its counterparts in other species within the family. The functionality differences of the 3 proteins are poorly understood. In the present study, 3 PBP cDNAs (SinfPBP1, 2,3) were identified from the pink rice borer, Sesamia inferens, for the first time. The quantitative real-time PCR indicated that the 3 PBPs displayed similar temporal but very different sex related expression profiles. Expression of SinfPBP1 and SinfPBP2 were highly and moderately male biased, respectively, while SinfPBP3 was slightly female biased, as SinfPBPs were expressed at very different levels (PBP1 > PBP2 >> PBP3) in male antennae, but at similar levels in female antennae. Furthermore, the 3 SinfPBPs displayed different ligand binding profiles in fluorescence competitive binding assays. SinfPBP1 exhibited high and similar binding affinities to all 3 sex pheromone components (K-i = 0.72-1.60 mu M), while SinfPBP2 showed selective binding to the alcohol and aldehyde components (K-i = 0.78-1.71 mu M), and SinfPBP3 showed no obvious binding to the 3 sex pheromone components. The results suggest that SinfPBP1 plays a major role in the reception of female sex pheromones in S. inferens, while SinfPBP3 plays a least role (if any) and SinfPBP2 functions as a recognizer of alcohol and aldehyde components. (C) 2014 Elsevier Ltd. All rights reserved.