A DISEASE RESISTANCE GENE IN ARABIDOPSIS WITH SPECIFICITY FOR 2 DIFFERENT PATHOGEN AVIRULENCE GENES
A DISEASE RESISTANCE GENE IN ARABIDOPSIS WITH SPECIFICITY FOR 2 DIFFERENT PATHOGEN AVIRULENCE GENES
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DOI：
10.1105/tpc.6.7.927
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发表时间：
1994-07-01
期刊：
影响因子：
11.6
通讯作者：
INNES, RW
中科院分区：
文献类型：
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作者：
BISGROVE, SR;SIMONICH, MT;INNES, RW
拟南芥的RPS3和RPM1抗病基因分别对携带avrB和avrRpm1无毒基因的丁香假单胞菌产生抗性。我们之前已经证明RPS3和RPM1在基因上是紧密联系的。在这里，我们证明了RPS3和RPM1实际上是同一个基因。我们用一株携带avrB基因的紫丁香R菌株筛选了一个诱变拟南芥群体，发现了12个敏感突变体。所有12个突变体都对携带avrRpm1的同基因菌株敏感，这表明RPS3和RPM1功能都丧失了。未发现仅丧失RPS3功能的突变体。对四个独立突变体的遗传分析表明，这些损伤位于RPS3中，因此，拟南芥中的一个基因赋予了对两个不同的病原菌无毒基因的抗性-基因对基因的相互作用。这一观察表明，RPS3/RPM1基因产物可以结合多个病原体配体，或者，它不起到受体的作用。
The RPS3 and RPM1 disease resistance loci of Arabidopsis confer resistance to Pseudomonas syringae strains that carry the avirulence genes avrB and avrRpm1, respectively. We have previously shown that RPS3 and RPM1 are closely linked genetically. Here, we show that RPS3 and RPM1 are in fact the same gene. We screened a mutagenized Arabidopsis population with a R syringae strain carrying avrB and found 12 susceptible mutants. All 12 mutants were also susceptible to an isogenic strain carrying avrRpm1, indicating a loss of both RPS3 and RPM1 functions. No mutants were recovered that lost only RPS3 function. Genetic analysis of four independent mutants revealed that the lesions were in RPS3 Thus, a single gene in Arabidopsis confers resistance that is specific to two distinct pathogen avirulence genes - a gene-for-genes interaction. This observation suggests that the RPS3/RPM1 gene product can bind multiple pathogen ligands, or alternatively, that it does not function as a receptor.