Investigation of varicella-zoster virus variation by heteroduplex mobility assay.
Investigation of varicella-zoster virus variation by heteroduplex mobility assay.
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通过异源双链体迁移试验研究水痘带状疱疹病毒变异。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
J. Breuer
中科院分区：
文献类型：
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作者：
W. Barrett;K. Hawrami;J. Clarke;J. Breuer
异源双链体迁移率测定的37个地区进行了10个英国分离株水痘带状疱疹病毒，4例带状疱疹，6例水痘。分离株之间的变异为0.061%，比人类疱疹病毒家族的任何其他成员低至少5倍。在37个检测区域中，有15个区域有29个单核苷酸多态性，超过一半的多态性位于4个基因片段中。在29个SNP中，11个是非同义的，并且这些被聚集在6个基因中。从一个孩子和她母亲身上分离出来的病毒，在每个检测位点都是相同的。所有其他病毒可以通过重复区域R1、R2和R5的SNP和长度多态性的组合来区分。
Heteroduplex mobility assays of 37 regions were performed on ten UK isolates of varicella zoster virus, four from cases of zoster, and six from cases of chickenpox. The variation between isolates was found to be 0.061%, which is at least five times lower than any other member of the human herpesvirus family. Fifteen of the 37 regions tested had 29 single nucleotide polymorphisms, and over half the polymorphisms were located in four gene fragments. Of the 29 SNPs, eleven were non-synonymous and these were clustered in six genes. Isolates from a child and her mother to whom she had transmitted the virus, were identical at every locus tested. All other viruses could be distinguished by a combination of SNPs and length polymorphisms of the repeat regions R1, R2 and R5.
DOI：
10.1073/pnas.90.21.10325
发表时间：
1993-11-01
影响因子：
11.1
作者：
GANGULY, A;ROCK, MJ;PROCKOP, DJ
通讯作者：
PROCKOP, DJ